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PCMD-1 bridges the centrioles and the PCM scaffold in C. elegans

Lisa Stenzel, Judith Mehler, Alina Schreiner, Sim Üstüner, Elisa Zuccoli, View ORCID ProfileEsther Zanin, View ORCID ProfileTamara Mikeladze-Dvali
doi: https://doi.org/10.1101/2020.11.09.375865
Lisa Stenzel
1Department Biology II, Ludwig-Maximilians University, Munich, 82152 Planegg-Martinsried, Germany
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Judith Mehler
1Department Biology II, Ludwig-Maximilians University, Munich, 82152 Planegg-Martinsried, Germany
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Alina Schreiner
1Department Biology II, Ludwig-Maximilians University, Munich, 82152 Planegg-Martinsried, Germany
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Sim Üstüner
1Department Biology II, Ludwig-Maximilians University, Munich, 82152 Planegg-Martinsried, Germany
2Institut for Moleculare Botany, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany
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Elisa Zuccoli
1Department Biology II, Ludwig-Maximilians University, Munich, 82152 Planegg-Martinsried, Germany
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Esther Zanin
1Department Biology II, Ludwig-Maximilians University, Munich, 82152 Planegg-Martinsried, Germany
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Tamara Mikeladze-Dvali
1Department Biology II, Ludwig-Maximilians University, Munich, 82152 Planegg-Martinsried, Germany
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  • ORCID record for Tamara Mikeladze-Dvali
  • For correspondence: tmdvali@bio.lmu.de
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ABSTRACT

Correct cell division relies on the formation of a bipolar spindle. In animal cells, microtubule nucleation at the spindle poles is facilitated by the pericentriolar material (PCM), which assembles around a pair of centrioles. Although centrioles are essential for PCM assembly, proteins that anchor the PCM to the centrioles are less known. Here we investigate the molecular function of PCMD-1 in bridging the PCM and the centrioles in Caenorhabditis elegans.

We demonstrate that centrosomal recruitment of PCMD-1 is dependent on the outer centriolar protein SAS-7. While the most C-terminal part of PCMD-1 is sufficient to target it to the centrosome, the coiled-coil domain promotes its accumulation by facilitating self-interaction. We reveal that PCMD-1 is bridging the centrioles and PCM scaffold through protein-protein interactions with the PCM scaffold protein SPD-5, the mitotic kinase PLK-1 and the centriolar protein SAS-4. Using an ectopic translocation assay, we show that PCMD-1 is able to selectively recruit downstream PCM scaffold components to an ectopic location in the cell, indicating that PCMD-1 is sufficient to anchor the PCM scaffold proteins to the centrioles. Our work suggests that PCMD-1 is an essential functional bridge between the centrioles and the PCM.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 10, 2020.
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PCMD-1 bridges the centrioles and the PCM scaffold in C. elegans
Lisa Stenzel, Judith Mehler, Alina Schreiner, Sim Üstüner, Elisa Zuccoli, Esther Zanin, Tamara Mikeladze-Dvali
bioRxiv 2020.11.09.375865; doi: https://doi.org/10.1101/2020.11.09.375865
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PCMD-1 bridges the centrioles and the PCM scaffold in C. elegans
Lisa Stenzel, Judith Mehler, Alina Schreiner, Sim Üstüner, Elisa Zuccoli, Esther Zanin, Tamara Mikeladze-Dvali
bioRxiv 2020.11.09.375865; doi: https://doi.org/10.1101/2020.11.09.375865

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