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- December 18, 2020.
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- Version 1 (November 10, 2020 - 09:32).
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Author Information
- Alison Schroer Vander Roest*,1,
- Chao Liu*,2,
- Makenna M Morck2,
- Kristina Bezold Kooiker1,3,
- Gwanghyun Jung1,
- Dan Song2,
- Aminah Dawood2,
- Arnav Jhingran1,
- Gaspard Pardon4,
- Sara Ranjbarvaziri1,
- Giovanni Fajardo1,
- Mingming Zhao1,
- Kenneth S Campbell5,
- Beth L Pruitt6,
- James A Spudich2,**,
- Kathleen M Ruppel2 and
- Daniel Bernstein1
- 1Pediatrics (Cardiology), Stanford University, 2200 Biomedical Innovations Building, 240 Pasteur Dr., Palo Alto CA, 94304
- 2Biochemistry, Stanford University, 405 Beckman Center, 279 Campus Dr. West, Stanford CA 94305
- 3School of Medicine, University of Washington, UW Medicine at South Lake Union, 850 Republican Street, Box 358056, Seattle, WA 98109
- 4Departments of Mechanical Engineering and Bioengineering, Stanford University, School of Engineering and School of Medicine, Stanford, CA, USA
- 5Department of Physiology and Division of Cardiovascular Medicine, University of Kentucky, MS508, 800 Rose Street, Lexington, KY 40536
- 6Bioengineering, University of California, Santa Barbara, Room 3108, University of California, Santa Barbara, Santa Barbara, CA 93106-5070
- ↵**James A Spudich, Ph.D., 405 Beckman Center, 279 Campus Dr. West, Stanford CA 94305, 650-723-7634, Email:
jspudich{at}stanford.edu
↵* These authors contributed equally to this work
Author Contributions: AVR performed and analyzed the functional measurements of the hiPSC-CMs (TFM, immunostaining, inhibitor treatment) and performed computational modeling. CL developed techniques and performed and analyzed the HFS and motility measurements. MMM performed and analyzed the 2-hep and 25-hep ATPase and single-turnover measurements. KBK performed the initial gene editing of the cells and assisted with preliminary molecular and cellular measurements. CL, MMM, and DS prepared protein. DS performed preliminary motility measurements. GP wrote the code for analysis of the TFM data. GJ contributed to the quantification of cell hypertrophy. GJ, SR, GF, and MZ assisted with functional measurements of hiPSC-CMs. AJ contributed to the sensitivity analysis and optimization of the modeling results. KSC developed the original computer model and assisted with its adaptation for this project. KMR and AD prepared the transgenic virus. AVR, CL, and MMM wrote the manuscript. BLP, KMR, JAS, and DB contributed to intellectual oversight of the project and writing the manuscript. All authors contributed intellectually to discussions of the results and reviewed and edited the paper.