Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Glia actively sculpt sensory neurons by controlled phagocytosis to tune animal behavior

Stephan Raiders, Erik Calvin Black, Andrea Bae, Stephen MacFarlane, Shai Shaham, View ORCID ProfileAakanksha Singhvi
doi: https://doi.org/10.1101/2020.11.11.378893
Stephan Raiders
1Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA
2Molecular and Cellular Biology Graduate Program, University of Washington-Seattle WA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Erik Calvin Black
1Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andrea Bae
3Laboratory of Developmental Genetics, The Rockefeller University, New York, NY
4Dominick P. Purpura Department of Neuroscience, Albert Einstein College of Medicine, NY
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephen MacFarlane
5Cellular Imaging Shared Resources, Fred Hutchinson Cancer Research Center, Seattle, WA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shai Shaham
3Laboratory of Developmental Genetics, The Rockefeller University, New York, NY
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aakanksha Singhvi
1Division of Basic Sciences, Fred Hutchinson Cancer Research Center, Seattle, WA
2Molecular and Cellular Biology Graduate Program, University of Washington-Seattle WA
6Department of Biological Structure, University of Washington School of Medicine, WA
7Brotman Baty Institute for Precision Medicine, Seattle WA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Aakanksha Singhvi
  • For correspondence: asinghvi@fredhutch.org
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

ABSTRACT

Glia in the central nervous system engulf neuron fragments during synapse remodeling and recycling of photoreceptor outer-segments. Whether glia passively clear shed neuronal debris, or actively remove neuron fragments is unknown. How pruning of single-neuron endings impacts animal behavior is also unclear. Here we report that adult C. elegans AMsh glia engulf sensory endings of the AFD thermosensory neuron. Engulfment is regulated by temperature, AFD’s sensory input, and tracks AFD activity. Phosphatidylserine (PS) flippase TAT-1/ATP8A, functions with glial PS-receptor PSR-1/PSR and PAT-2/α-integrin to initiate engulfment. Glial CED-10/Rac1 GTPase, acting through a conserved GEF complex, executes phagocytosis using the actin-remodeler WSP-1/nWASp and the membrane-sealing factor EFF-1 fusogen. CED-10 levels determine engulfment rates, and engulfment-defective mutants exhibit altered AFD-ending shape and thermosensory behavior. Our findings reveal a molecular pathway underpinning glia-dependent phagocytosis in a peripheral sense-organ, and demonstrate that glia actively engulf neuron-fragments, with profound consequences on neuron shape and animal behavior.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted November 12, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Glia actively sculpt sensory neurons by controlled phagocytosis to tune animal behavior
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Glia actively sculpt sensory neurons by controlled phagocytosis to tune animal behavior
Stephan Raiders, Erik Calvin Black, Andrea Bae, Stephen MacFarlane, Shai Shaham, Aakanksha Singhvi
bioRxiv 2020.11.11.378893; doi: https://doi.org/10.1101/2020.11.11.378893
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Glia actively sculpt sensory neurons by controlled phagocytosis to tune animal behavior
Stephan Raiders, Erik Calvin Black, Andrea Bae, Stephen MacFarlane, Shai Shaham, Aakanksha Singhvi
bioRxiv 2020.11.11.378893; doi: https://doi.org/10.1101/2020.11.11.378893

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Neuroscience
Subject Areas
All Articles
  • Animal Behavior and Cognition (4234)
  • Biochemistry (9128)
  • Bioengineering (6774)
  • Bioinformatics (23989)
  • Biophysics (12117)
  • Cancer Biology (9523)
  • Cell Biology (13775)
  • Clinical Trials (138)
  • Developmental Biology (7630)
  • Ecology (11686)
  • Epidemiology (2066)
  • Evolutionary Biology (15506)
  • Genetics (10638)
  • Genomics (14322)
  • Immunology (9479)
  • Microbiology (22832)
  • Molecular Biology (9089)
  • Neuroscience (48987)
  • Paleontology (355)
  • Pathology (1480)
  • Pharmacology and Toxicology (2568)
  • Physiology (3844)
  • Plant Biology (8327)
  • Scientific Communication and Education (1471)
  • Synthetic Biology (2296)
  • Systems Biology (6187)
  • Zoology (1300)