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Functional correlates of immediate early gene expression in mouse visual cortex

David Mahringer, Pawel Zmarz, Hiroyuki Okuno, Haruhiko Bito, Georg B. Keller
doi: https://doi.org/10.1101/2020.11.12.379909
David Mahringer
1Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
2Faculty of Natural Sciences, University of Basel, Basel, Switzerland
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Pawel Zmarz
1Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
2Faculty of Natural Sciences, University of Basel, Basel, Switzerland
3Department of Organismic and Evolutionary Biology and Center for Brain Science, Harvard University, Cambridge, MA 02138, USA
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Hiroyuki Okuno
4Department of Biochemistry and Molecular Biology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Kagoshima 890-8544, Japan
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Haruhiko Bito
5Department of Neurochemistry, Graduate School of Medicine, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan
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Georg B. Keller
1Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
2Faculty of Natural Sciences, University of Basel, Basel, Switzerland
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  • For correspondence: georg.keller@fmi.ch
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Abstract

The expression of immediate early genes (IEGs) in visual cortex can be triggered by visual input and is associated with certain forms of neuronal plasticity. How IEG expression in cortical neurons relates to neuronal activity or experience-dependent changes of neuronal activity, however, is still unclear. Using three transgenic mouse lines that express GFP under the control of different IEG promoters (c-fos, egr1 or Arc), we recorded both neuronal activity and IEG expression levels in primary visual cortex before and after a mouse’s first visual exposure, and subsequent visuomotor learning. We found that expression levels of all three IEGs correlated positively with neuronal activity, and that different IEGs are preferentially expressed in different functional cell types. Neurons with strong motor-related activity preferentially expressed EGR1 while neurons that developed strong visually driven activity preferentially expressed Arc. Our findings suggest that during functional development of visual cortex different IEGs are preferentially expressed in neurons that receive different functional types of input.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted November 12, 2020.
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Functional correlates of immediate early gene expression in mouse visual cortex
David Mahringer, Pawel Zmarz, Hiroyuki Okuno, Haruhiko Bito, Georg B. Keller
bioRxiv 2020.11.12.379909; doi: https://doi.org/10.1101/2020.11.12.379909
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Functional correlates of immediate early gene expression in mouse visual cortex
David Mahringer, Pawel Zmarz, Hiroyuki Okuno, Haruhiko Bito, Georg B. Keller
bioRxiv 2020.11.12.379909; doi: https://doi.org/10.1101/2020.11.12.379909

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