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Homemade Bread: Repurposing an Ancient Technology for Low Cost in vitro Tissue Engineering

Jessica T. Holmes, Ziba Jaberansari, William Collins, Maxime Leblanc Latour, Daniel J. Modulevsky, View ORCID ProfileAndrew E. Pelling
doi: https://doi.org/10.1101/2020.11.13.353698
Jessica T. Holmes
1Department of Physics, STEM Complex, 150 Louis Pasteur Pvt., University of Ottawa, Ottawa, ON, K1N5N5 Canada
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Ziba Jaberansari
1Department of Physics, STEM Complex, 150 Louis Pasteur Pvt., University of Ottawa, Ottawa, ON, K1N5N5 Canada
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William Collins
1Department of Physics, STEM Complex, 150 Louis Pasteur Pvt., University of Ottawa, Ottawa, ON, K1N5N5 Canada
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Maxime Leblanc Latour
1Department of Physics, STEM Complex, 150 Louis Pasteur Pvt., University of Ottawa, Ottawa, ON, K1N5N5 Canada
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Daniel J. Modulevsky
2Department of Biology, Gendron Hall, 30 Marie Curie, University of Ottawa, Ottawa, ON, K1N5N5 Canada
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Andrew E. Pelling
1Department of Physics, STEM Complex, 150 Louis Pasteur Pvt., University of Ottawa, Ottawa, ON, K1N5N5 Canada
2Department of Biology, Gendron Hall, 30 Marie Curie, University of Ottawa, Ottawa, ON, K1N5N5 Canada
3Institute for Science Society and Policy, Simard Hall, 60 University, University of Ottawa, Ottawa, ON, K1N5N5 Canada
4SymbioticA, School of Human Sciences, University of Western Australia, Perth, WA, 6009 Australia
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  • ORCID record for Andrew E. Pelling
  • For correspondence: a@pellinglab.net
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ABSTRACT

Cellular function is well known to be influenced by the physical cues and architecture of their three dimensional (3D) microenvironment. As such, numerous synthetic and naturally-occurring biomaterials have been developed to provide such architectures to support the proliferation of mammalian cells in vitro and in vivo. In recent years, our group, and others, have shown that scaffolds derived from plants can be utilized for tissue engineering applications in biomedicine and in the burgeoning cultured meat industry. Such scaffolds are straightforward to prepare, allowing researchers to take advantage of their intrinsic 3D microarchitectures. During the 2020 SARS-CoV-2 pandemic many people around the world began to rediscover the joy of preparing bread at home and as a research group, our members participated in this trend. Having observed the high porosity of the crumb (the internal portion of the bread) we were inspired to investigate whether it might support the proliferation of mammalian cells in vitro. Here, we develop and validate a yeast-free “soda bread” that maintains its mechanical stability over two weeks in culture conditions. The scaffolding is highly porous, allowing the 3D proliferation of multiple cell types relevant to both biomedical tissue engineering and the development of novel future foods. Bread derived scaffolds are highly scalable and represent a surprising new alternative to synthetic or animal-derived scaffolds for addressing a diverse variety of tissue engineering challenges.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 13, 2020.
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Homemade Bread: Repurposing an Ancient Technology for Low Cost in vitro Tissue Engineering
Jessica T. Holmes, Ziba Jaberansari, William Collins, Maxime Leblanc Latour, Daniel J. Modulevsky, Andrew E. Pelling
bioRxiv 2020.11.13.353698; doi: https://doi.org/10.1101/2020.11.13.353698
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Homemade Bread: Repurposing an Ancient Technology for Low Cost in vitro Tissue Engineering
Jessica T. Holmes, Ziba Jaberansari, William Collins, Maxime Leblanc Latour, Daniel J. Modulevsky, Andrew E. Pelling
bioRxiv 2020.11.13.353698; doi: https://doi.org/10.1101/2020.11.13.353698

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