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Analysis of SARS-CoV-2 spike glycosylation reveals shedding of a vaccine candidate

View ORCID ProfileJuliane Brun, View ORCID ProfileSnežana Vasiljevic, View ORCID ProfileBevin Gangadharan, View ORCID ProfileMario Hensen, View ORCID ProfileAnu V. Chandran, View ORCID ProfileMichelle L. Hill, View ORCID ProfileJ.L. Kiappes, View ORCID ProfileRaymond A. Dwek, View ORCID ProfileDominic S. Alonzi, View ORCID ProfileWeston B. Struwe, View ORCID ProfileNicole Zitzmann
doi: https://doi.org/10.1101/2020.11.16.384594
Juliane Brun
1Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom
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Snežana Vasiljevic
1Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom
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  • ORCID record for Snežana Vasiljevic
Bevin Gangadharan
1Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom
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Mario Hensen
1Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom
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Anu V. Chandran
1Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom
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Michelle L. Hill
1Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom
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  • ORCID record for Michelle L. Hill
J.L. Kiappes
1Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom
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Raymond A. Dwek
1Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom
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  • ORCID record for Raymond A. Dwek
Dominic S. Alonzi
1Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom
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Weston B. Struwe
2Physical and Theoretical Chemistry Laboratory, Department of Chemistry, University of Oxford, United Kingdom
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  • ORCID record for Weston B. Struwe
  • For correspondence: weston.struwe@chem.ox.ac.uk nicole.zitzmann@bioch.ox.ac.uk
Nicole Zitzmann
1Oxford Glycobiology Institute, Department of Biochemistry, University of Oxford, Oxford, United Kingdom
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  • ORCID record for Nicole Zitzmann
  • For correspondence: weston.struwe@chem.ox.ac.uk nicole.zitzmann@bioch.ox.ac.uk
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Abstract

Severe acute respiratory syndrome coronavirus 2 is the causative pathogen of the COVID-19 pandemic which as of Nov 15, 2020 has claimed 1,319,946 lives worldwide. Vaccine development focuses on the viral trimeric spike glycoprotein as the main target of the humoral immune response. Viral spikes carry glycans that facilitate immune evasion by shielding specific protein epitopes from antibody neutralisation. Immunogen integrity is therefore important for glycoprotein-based vaccine candidates. Here we show how site-specific glycosylation differs between virus-derived spikes and spike proteins derived from a viral vectored SARS-CoV-2 vaccine candidate. We show that their distinctive cellular secretion pathways result in different protein glycosylation and secretion patterns, which may have implications for the resulting immune response and future vaccine design.

Competing Interest Statement

W.B.S. is a shareholder and consultant to Refeyn Ltd. All other authors declare no conflict of interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted November 16, 2020.
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Analysis of SARS-CoV-2 spike glycosylation reveals shedding of a vaccine candidate
Juliane Brun, Snežana Vasiljevic, Bevin Gangadharan, Mario Hensen, Anu V. Chandran, Michelle L. Hill, J.L. Kiappes, Raymond A. Dwek, Dominic S. Alonzi, Weston B. Struwe, Nicole Zitzmann
bioRxiv 2020.11.16.384594; doi: https://doi.org/10.1101/2020.11.16.384594
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Analysis of SARS-CoV-2 spike glycosylation reveals shedding of a vaccine candidate
Juliane Brun, Snežana Vasiljevic, Bevin Gangadharan, Mario Hensen, Anu V. Chandran, Michelle L. Hill, J.L. Kiappes, Raymond A. Dwek, Dominic S. Alonzi, Weston B. Struwe, Nicole Zitzmann
bioRxiv 2020.11.16.384594; doi: https://doi.org/10.1101/2020.11.16.384594

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