Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Immunogenicity and protective efficacy of one- and two-dose regimens of the Ad26.COV2.S COVID-19 vaccine candidate in adult and aged rhesus macaques

View ORCID ProfileLaura Solforosi, Harmjan Kuipers, Sietske K. Rosendahl Huber, View ORCID ProfileJoan E.M. van der Lubbe, Liesbeth Dekking, Dominika N. Czapska-Casey, Ana Izquierdo Gil, Miranda R.M. Baert, Joke Drijver, Joost Vaneman, Ella van Huizen, Ying Choi, Jessica Vreugdenhil, Sanne Kroos, Adriaan H. de Wilde, Eleni Kourkouta, Jerome Custers, View ORCID ProfileTim J. Dalebout, View ORCID ProfileSebenzile K. Myeni, View ORCID ProfileMarjolein Kikkert, View ORCID ProfileEric J. Snijder, View ORCID ProfileDan H. Barouch, View ORCID ProfileKinga P. Böszörményi, Marieke A. Stammes, Ivanela Kondova, Ernst J. Verschoor, Babs E. Verstrepen, View ORCID ProfileGerrit Koopman, View ORCID ProfilePetra Mooij, View ORCID ProfileWilly M.J.M. Bogers, Marjolein van Heerden, View ORCID ProfileLeacky Muchene, Jeroen T.B.M. Tolboom, View ORCID ProfileRamon Roozendaal, View ORCID ProfileHanneke Schuitemaker, View ORCID ProfileFrank Wegmann, Roland C. Zahn
doi: https://doi.org/10.1101/2020.11.17.368258
Laura Solforosi
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Laura Solforosi
Harmjan Kuipers
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sietske K. Rosendahl Huber
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joan E.M. van der Lubbe
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Joan E.M. van der Lubbe
Liesbeth Dekking
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dominika N. Czapska-Casey
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ana Izquierdo Gil
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Miranda R.M. Baert
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joke Drijver
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joost Vaneman
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ella van Huizen
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ying Choi
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jessica Vreugdenhil
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sanne Kroos
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Adriaan H. de Wilde
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eleni Kourkouta
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jerome Custers
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tim J. Dalebout
2Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Tim J. Dalebout
Sebenzile K. Myeni
2Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sebenzile K. Myeni
Marjolein Kikkert
2Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Marjolein Kikkert
Eric J. Snijder
2Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Eric J. Snijder
Dan H. Barouch
3Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Dan H. Barouch
Kinga P. Böszörményi
4Biomedical Primate Research Centre, Rijswijk, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Kinga P. Böszörményi
Marieke A. Stammes
4Biomedical Primate Research Centre, Rijswijk, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ivanela Kondova
4Biomedical Primate Research Centre, Rijswijk, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ernst J. Verschoor
4Biomedical Primate Research Centre, Rijswijk, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Babs E. Verstrepen
4Biomedical Primate Research Centre, Rijswijk, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gerrit Koopman
4Biomedical Primate Research Centre, Rijswijk, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Gerrit Koopman
Petra Mooij
4Biomedical Primate Research Centre, Rijswijk, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Petra Mooij
Willy M.J.M. Bogers
4Biomedical Primate Research Centre, Rijswijk, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Willy M.J.M. Bogers
Marjolein van Heerden
5Non-Clinical Safety Toxicology/Pathology, Janssen Research and Development, Beerse, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Leacky Muchene
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Leacky Muchene
Jeroen T.B.M. Tolboom
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ramon Roozendaal
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ramon Roozendaal
Hanneke Schuitemaker
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Hanneke Schuitemaker
Frank Wegmann
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Frank Wegmann
Roland C. Zahn
1Janssen Vaccines and Prevention B.V., Leiden, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: rzahn@its.jnj.com
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Safe and effective coronavirus disease (COVID)-19 vaccines are urgently needed to control the ongoing pandemic. While single-dose vaccine regimens would provide multiple advantages, two doses may improve the magnitude and durability of immunity and protective efficacy. We assessed one- and two-dose regimens of the Ad26.COV2.S vaccine candidate in adult and aged non-human primates (NHP). A two-dose Ad26.COV2.S regimen induced higher peak binding and neutralizing antibody responses compared to a single dose. In one-dose regimens neutralizing antibody responses were stable for at least 14 weeks, providing an early indication of durability. Ad26.COV2.S induced humoral immunity and Th1 skewed cellular responses in aged NHP that were comparable to adult animals. Importantly, aged Ad26.COV2.S-vaccinated animals challenged 3 months post -dose 1 with a SARS-CoV-2 spike G614 variant showed near complete lower and substantial upper respiratory tract protection for both regimens. These are the first NHP data showing COVID-19 vaccine protection against the SARS-CoV-2 spike G614 variant and support ongoing clinical Ad26.COV2.S development.

Summary COVID-19 vaccines are urgently needed and while single-dose vaccines are preferred, two-dose regimens may improve efficacy. We show improved Ad26.COV2.S immunogenicity in non-human primates after a second vaccine dose, while both regimens protected aged animals against SARS-CoV-2 disease.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Protective efficacy of one- and two-dose Ad26.COV2.S vaccine regimes in aged rhesus macaques Figure 4 and figure 5 are new figures relative to the added efficacy data Supplementary figure 5 and supplementary figure 6 are new figures relative to the added efficacy data

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted January 04, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Immunogenicity and protective efficacy of one- and two-dose regimens of the Ad26.COV2.S COVID-19 vaccine candidate in adult and aged rhesus macaques
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Immunogenicity and protective efficacy of one- and two-dose regimens of the Ad26.COV2.S COVID-19 vaccine candidate in adult and aged rhesus macaques
Laura Solforosi, Harmjan Kuipers, Sietske K. Rosendahl Huber, Joan E.M. van der Lubbe, Liesbeth Dekking, Dominika N. Czapska-Casey, Ana Izquierdo Gil, Miranda R.M. Baert, Joke Drijver, Joost Vaneman, Ella van Huizen, Ying Choi, Jessica Vreugdenhil, Sanne Kroos, Adriaan H. de Wilde, Eleni Kourkouta, Jerome Custers, Tim J. Dalebout, Sebenzile K. Myeni, Marjolein Kikkert, Eric J. Snijder, Dan H. Barouch, Kinga P. Böszörményi, Marieke A. Stammes, Ivanela Kondova, Ernst J. Verschoor, Babs E. Verstrepen, Gerrit Koopman, Petra Mooij, Willy M.J.M. Bogers, Marjolein van Heerden, Leacky Muchene, Jeroen T.B.M. Tolboom, Ramon Roozendaal, Hanneke Schuitemaker, Frank Wegmann, Roland C. Zahn
bioRxiv 2020.11.17.368258; doi: https://doi.org/10.1101/2020.11.17.368258
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Immunogenicity and protective efficacy of one- and two-dose regimens of the Ad26.COV2.S COVID-19 vaccine candidate in adult and aged rhesus macaques
Laura Solforosi, Harmjan Kuipers, Sietske K. Rosendahl Huber, Joan E.M. van der Lubbe, Liesbeth Dekking, Dominika N. Czapska-Casey, Ana Izquierdo Gil, Miranda R.M. Baert, Joke Drijver, Joost Vaneman, Ella van Huizen, Ying Choi, Jessica Vreugdenhil, Sanne Kroos, Adriaan H. de Wilde, Eleni Kourkouta, Jerome Custers, Tim J. Dalebout, Sebenzile K. Myeni, Marjolein Kikkert, Eric J. Snijder, Dan H. Barouch, Kinga P. Böszörményi, Marieke A. Stammes, Ivanela Kondova, Ernst J. Verschoor, Babs E. Verstrepen, Gerrit Koopman, Petra Mooij, Willy M.J.M. Bogers, Marjolein van Heerden, Leacky Muchene, Jeroen T.B.M. Tolboom, Ramon Roozendaal, Hanneke Schuitemaker, Frank Wegmann, Roland C. Zahn
bioRxiv 2020.11.17.368258; doi: https://doi.org/10.1101/2020.11.17.368258

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Immunology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3686)
  • Biochemistry (7767)
  • Bioengineering (5666)
  • Bioinformatics (21237)
  • Biophysics (10553)
  • Cancer Biology (8159)
  • Cell Biology (11905)
  • Clinical Trials (138)
  • Developmental Biology (6737)
  • Ecology (10388)
  • Epidemiology (2065)
  • Evolutionary Biology (13838)
  • Genetics (9694)
  • Genomics (13054)
  • Immunology (8121)
  • Microbiology (19936)
  • Molecular Biology (7825)
  • Neuroscience (42959)
  • Paleontology (318)
  • Pathology (1276)
  • Pharmacology and Toxicology (2256)
  • Physiology (3350)
  • Plant Biology (7207)
  • Scientific Communication and Education (1309)
  • Synthetic Biology (1998)
  • Systems Biology (5528)
  • Zoology (1126)