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High-throughput phenotyping methods for quantifying hair fiber morphology

View ORCID ProfileTina Lasisi, View ORCID ProfileArslan A. Zaidi, View ORCID ProfileTimothy Harding Webster, View ORCID ProfileNicholas Bradley Stephens, Kendall Routch, View ORCID ProfileNina Grace Jablonski, View ORCID ProfileMark David Shriver
doi: https://doi.org/10.1101/2020.11.24.392191
Tina Lasisi
1Department of Anthropology, Penn State University
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  • For correspondence: tina.lasisi@gmail.com
Arslan A. Zaidi
2Department of Genetics, Perelman School of Medicine, University of Pennsylvania
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Timothy Harding Webster
3Department of Anthropology, University of Utah
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Nicholas Bradley Stephens
1Department of Anthropology, Penn State University
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Kendall Routch
1Department of Anthropology, Penn State University
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Nina Grace Jablonski
1Department of Anthropology, Penn State University
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Mark David Shriver
1Department of Anthropology, Penn State University
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Abstract

Quantifying the continuous variation in human scalp hair morphology is of interest to anthropologists, geneticists, dermatologists and forensic scientists, but existing methods for studying hair form are time-consuming and not widely used. Here, we present a high-throughput sample preparation protocol for the imaging of both longitudinal (curvature) and cross-sectional scalp hair morphology. Additionally, we describe and validate a new Python package designed to process longitudinal and cross-sectional hair images, segment them, and provide measurements of interest. Lastly, we apply our methods to an admixed African-European sample (n=140), demonstrating the benefit of quantifying hair morphology over qualitative classification or racial categories, and providing evidence against the long-held belief that cross-sectional morphology predicts curvature.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://github.com/tinalasisi/2020_HairPheno_manuscript

  • https://github.com/tinalasisi/fibermorph

  • https://pypi.org/project/fibermorph/

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted November 24, 2020.
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High-throughput phenotyping methods for quantifying hair fiber morphology
Tina Lasisi, Arslan A. Zaidi, Timothy Harding Webster, Nicholas Bradley Stephens, Kendall Routch, Nina Grace Jablonski, Mark David Shriver
bioRxiv 2020.11.24.392191; doi: https://doi.org/10.1101/2020.11.24.392191
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High-throughput phenotyping methods for quantifying hair fiber morphology
Tina Lasisi, Arslan A. Zaidi, Timothy Harding Webster, Nicholas Bradley Stephens, Kendall Routch, Nina Grace Jablonski, Mark David Shriver
bioRxiv 2020.11.24.392191; doi: https://doi.org/10.1101/2020.11.24.392191

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