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pH fluctuations drive waves of stereotypical cellular reorganizations during entry into quiescence

Basile Jacquel, View ORCID ProfileThéo Aspert, View ORCID ProfileDamien Laporte, View ORCID ProfileIsabelle Sagot, View ORCID ProfileGilles Charvin
doi: https://doi.org/10.1101/2020.11.25.395608
Basile Jacquel
1Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France
4Université de Strasbourg, Illkirch, France
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Théo Aspert
1Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France
4Université de Strasbourg, Illkirch, France
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Damien Laporte
5Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, Bordeaux, France
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Isabelle Sagot
5Institut de Biochimie et Génétique Cellulaires, CNRS UMR 5095, Bordeaux, France
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Gilles Charvin
1Department of Developmental Biology and Stem Cells, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France
2Centre National de la Recherche Scientifique, UMR7104, Illkirch, France
3Institut National de la Santé et de la Recherche Médicale, U964, Illkirch, France
4Université de Strasbourg, Illkirch, France
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  • ORCID record for Gilles Charvin
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Abstract

The life cycle of microorganisms is associated with dynamic metabolic transitions and complex cellular responses. In yeast, how metabolic signals control the progressive establishment of structural reorganizations observed in quiescent cells remains unclear. To address this question, we have developed a method that combines nutrient-limited proliferation assays at the population level with single-cell tracking to unravel the coordination between metabolic and structural transitions in cells during an unperturbed lifecycle. We show that non-monotonous internal pH fluctuations are in sync with successive waves of protein super-assemblies formation and ultimately lead to a cytosolic glass transition. Our results, therefore, suggest a simple model explaining how the complex developmental changes during the yeast life cycle are orchestrated by the sequence of metabolic transitions.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted November 26, 2020.
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pH fluctuations drive waves of stereotypical cellular reorganizations during entry into quiescence
Basile Jacquel, Théo Aspert, Damien Laporte, Isabelle Sagot, Gilles Charvin
bioRxiv 2020.11.25.395608; doi: https://doi.org/10.1101/2020.11.25.395608
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pH fluctuations drive waves of stereotypical cellular reorganizations during entry into quiescence
Basile Jacquel, Théo Aspert, Damien Laporte, Isabelle Sagot, Gilles Charvin
bioRxiv 2020.11.25.395608; doi: https://doi.org/10.1101/2020.11.25.395608

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