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Control of stem cell niche and fruit development in Arabidopsis thaliana by AGO10/ZWL requires the bHLH transcription factor INDEHISCENT

View ORCID ProfileManoj Valluru, Karim Sorefan
doi: https://doi.org/10.1101/2020.11.25.397513
Manoj Valluru
2The Medical School, University of Sheffield, Sheffield S10 2TN, United Kingdom
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  • For correspondence: m.valluru@sheffield.ac.uk
Karim Sorefan
1Faculty of Science, University of Sheffield, Sheffield S10 2TN, United Kingdom
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Abstract

Background The shoot apical meristem (SAM) in plants is composed of a small mound of pluripotent stem cells that generate new organs. ARGONAUTE10 (AGO10) is known to be critical for maintenance of the embryonic SAM by regulating the expression of Class III HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP III) transcription factors, which then modulate downstream responses to the key phytohormone auxin. However, we do not understand how AGO10 modulates auxin responses after embryogenesis in the mature plant.

Results Here we show that AGO10 regulates auxin responses in the post-embryonic SAM via the bHLH transcription factor INDEHISCENT (IND). IND directly regulates auxin responses in the SAM regulating the auxin transporter PIN1 via direct transcriptional regulation of PINOID kinase. We show that a loss of function ind mutation significantly restores ago10zwl-3 mutant SAM and fruit phenotypes. ago10zwl-3 mutants overexpress IND and overexpression of IND phenocopies the ago10zwl-3 SAM phenotypes, and regulates auxin transport and responses in the SAM. AGO10 also regulates post-embryonic development in the fruit via a similar genetic pathway.

Conclusions We characterise a molecular mechanism that is conserved during post embryonic development linking AGO10 directly to auxin responses.

Competing Interest Statement

The authors have declared no competing interest.

  • Abbreviations

    SAM
    Shoot apical meristem
    AGO10zwl-3
    ZWILLE-3 in Ler background.
    AGO10-4
    AGO10-4 in Col background.
    IND
    INDEHISCENT
    SPT
    SPATULA
    YFP
    yellow fluorescent protein
    GFP
    green fluorescent protein
    Ler
    Landsberg erecta
    qRT-PCR
    quantitative reverse transcriptase polymerase chain reaction
    HD-ZIP III
    HOMEODOMAIN LEUCINE ZIPPER class III
    REV
    REVOLUTA
    PHB
    PHABULOSA
    PHV
    PHAVALUTA
    PID
    PINOID
    GUS
    beta-Glucuronidase
    DEX
    dexamethasone
    GR
    glucocorticoid receptor
    ChIP
    Chromatin immunoprecipitation
    NPA
    Naphthylphthalamic acid
    GSEA
    gene set enrichment analysis.
  • Copyright 
    The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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    Posted November 26, 2020.
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    Control of stem cell niche and fruit development in Arabidopsis thaliana by AGO10/ZWL requires the bHLH transcription factor INDEHISCENT
    Manoj Valluru, Karim Sorefan
    bioRxiv 2020.11.25.397513; doi: https://doi.org/10.1101/2020.11.25.397513
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    Control of stem cell niche and fruit development in Arabidopsis thaliana by AGO10/ZWL requires the bHLH transcription factor INDEHISCENT
    Manoj Valluru, Karim Sorefan
    bioRxiv 2020.11.25.397513; doi: https://doi.org/10.1101/2020.11.25.397513

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