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An adaptive optics module for deep tissue multiphoton imaging in vivo

View ORCID ProfileCristina Rodríguez, View ORCID ProfileAnderson Chen, View ORCID ProfileJosé A. Rivera, View ORCID ProfileManuel A. Mohr, View ORCID ProfileYajie Liang, Wenzhi Sun, View ORCID ProfileDaniel E. Milkie, View ORCID ProfileThomas G. Bifano, View ORCID ProfileXiaoke Chen, View ORCID ProfileNa Ji
doi: https://doi.org/10.1101/2020.11.25.397968
Cristina Rodríguez
1Department of Physics, University of California, Berkeley, CA, USA
2Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
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  • ORCID record for Cristina Rodríguez
Anderson Chen
2Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
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José A. Rivera
1Department of Physics, University of California, Berkeley, CA, USA
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Manuel A. Mohr
3Department of Biology, Stanford University, Stanford, CA, USA
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Yajie Liang
2Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
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Wenzhi Sun
2Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
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Daniel E. Milkie
2Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
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Thomas G. Bifano
4Department of Mechanical Engineering, Photonics Center, Boston University, Boston, MA, USA
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Xiaoke Chen
3Department of Biology, Stanford University, Stanford, CA, USA
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Na Ji
1Department of Physics, University of California, Berkeley, CA, USA
2Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA
5Department of Molecular and Cell Biology, University of California, Berkeley, CA, USA
6Helen Wills Neuroscience Institute, University of California, Berkeley, CA, USA
7Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA
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  • For correspondence: jina@berkeley.edu
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ABSTRACT

Understanding complex biological systems requires visualizing structures and processes deep within living organisms. We developed a compact adaptive optics module and incorporated it into two- and three-photon fluorescence microscopes, to measure and correct tissue-induced aberrations. We resolved synaptic structures in deep cortical and subcortical areas of the mouse brain, and demonstrated high-resolution imaging of neuronal structures and somatosensory-evoked calcium responses in the mouse spinal cord at unprecedented depths in vivo.

Competing Interest Statement

N.J. and Howard Hughes Medical Institute have filed patent applications that relate to the principle of frequency-multiplexed aberration measurement. T.G.B. has a financial interest in Boston Micromachines Corporation (BMC), which produced commercially the deformable mirror used in this work.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted November 26, 2020.
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An adaptive optics module for deep tissue multiphoton imaging in vivo
Cristina Rodríguez, Anderson Chen, José A. Rivera, Manuel A. Mohr, Yajie Liang, Wenzhi Sun, Daniel E. Milkie, Thomas G. Bifano, Xiaoke Chen, Na Ji
bioRxiv 2020.11.25.397968; doi: https://doi.org/10.1101/2020.11.25.397968
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An adaptive optics module for deep tissue multiphoton imaging in vivo
Cristina Rodríguez, Anderson Chen, José A. Rivera, Manuel A. Mohr, Yajie Liang, Wenzhi Sun, Daniel E. Milkie, Thomas G. Bifano, Xiaoke Chen, Na Ji
bioRxiv 2020.11.25.397968; doi: https://doi.org/10.1101/2020.11.25.397968

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