Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Multi-parameter formulation development for an HIV-vaccine protein with direct validation of epitope binding integrity and stoichiometry

Corinna Popp, Philipp Schramm, Ralf Wagner, David Peterhoff, Christopher Battle, Christian Kleusch, Maximilian G. Plach
doi: https://doi.org/10.1101/2020.11.30.403873
Corinna Popp
12bind GmbH, Regensburg, Germany /
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Philipp Schramm
2NanoTemper Technologies GmbH, Munich, Germany /
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ralf Wagner
3Molecular Microbiology (Virology), University of Regensburg, Germany /
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
David Peterhoff
3Molecular Microbiology (Virology), University of Regensburg, Germany /
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christopher Battle
2NanoTemper Technologies GmbH, Munich, Germany /
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christian Kleusch
2NanoTemper Technologies GmbH, Munich, Germany /
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: plach@2bind.com christian.kleusch@nanotempertech.com
Maximilian G. Plach
12bind GmbH, Regensburg, Germany /
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: plach@2bind.com christian.kleusch@nanotempertech.com
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Vaccines are on the front line-of-defense against infectious diseases, ranging from threats we are familiar with, including polio, tuberculosis, or HIV, to novel and emerging threats such as SARS-CoV-2. Successful development of new protein-based vaccines requires sophisticated and efficient development of storage and formulation conditions. Here we demonstrate the combined power of 2bind’s sophisticated buffer matrix FORMOscreen® and NanoTemper Technologies’ novel Prometheus Panta high-throughput Dynamic Light Scattering/Nano Differential Scanning Fluorimetry instrument. We show that this combination can comprehensively improve critical biophysical parameters for the HIV-1 vaccine BG505-SOSIP and find the optimal formulation condition with unmatched efficiency.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • ↵+ Shared first authors

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted November 30, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Multi-parameter formulation development for an HIV-vaccine protein with direct validation of epitope binding integrity and stoichiometry
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Multi-parameter formulation development for an HIV-vaccine protein with direct validation of epitope binding integrity and stoichiometry
Corinna Popp, Philipp Schramm, Ralf Wagner, David Peterhoff, Christopher Battle, Christian Kleusch, Maximilian G. Plach
bioRxiv 2020.11.30.403873; doi: https://doi.org/10.1101/2020.11.30.403873
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Multi-parameter formulation development for an HIV-vaccine protein with direct validation of epitope binding integrity and stoichiometry
Corinna Popp, Philipp Schramm, Ralf Wagner, David Peterhoff, Christopher Battle, Christian Kleusch, Maximilian G. Plach
bioRxiv 2020.11.30.403873; doi: https://doi.org/10.1101/2020.11.30.403873

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Biophysics
Subject Areas
All Articles
  • Animal Behavior and Cognition (3689)
  • Biochemistry (7789)
  • Bioengineering (5674)
  • Bioinformatics (21282)
  • Biophysics (10576)
  • Cancer Biology (8173)
  • Cell Biology (11937)
  • Clinical Trials (138)
  • Developmental Biology (6762)
  • Ecology (10401)
  • Epidemiology (2065)
  • Evolutionary Biology (13863)
  • Genetics (9708)
  • Genomics (13070)
  • Immunology (8139)
  • Microbiology (19983)
  • Molecular Biology (7842)
  • Neuroscience (43053)
  • Paleontology (319)
  • Pathology (1279)
  • Pharmacology and Toxicology (2258)
  • Physiology (3351)
  • Plant Biology (7232)
  • Scientific Communication and Education (1312)
  • Synthetic Biology (2004)
  • Systems Biology (5537)
  • Zoology (1128)