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Diversity of developing peripheral glia revealed by single cell RNA sequencing

View ORCID ProfileOE Tasdemir-Yilmaz, NR Druckenbrod, OO Olukoya, AR Yung, I Bastille, MF Pazyra-Murphy, A Sitko, EB Hale, S Vigneau, View ORCID ProfileAA Gimelbrant, P Kharchenko, LV Goodrich, RA Segal
doi: https://doi.org/10.1101/2020.12.04.411975
OE Tasdemir-Yilmaz
1Dana-Farber Cancer Institute
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NR Druckenbrod
2Harvard Medical School
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OO Olukoya
2Harvard Medical School
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AR Yung
2Harvard Medical School
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I Bastille
2Harvard Medical School
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MF Pazyra-Murphy
1Dana-Farber Cancer Institute
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A Sitko
2Harvard Medical School
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EB Hale
2Harvard Medical School
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S Vigneau
1Dana-Farber Cancer Institute
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AA Gimelbrant
1Dana-Farber Cancer Institute
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P Kharchenko
2Harvard Medical School
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LV Goodrich
2Harvard Medical School
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  • For correspondence: rosalind_segal@dfci.harvard.edu
RA Segal
2Harvard Medical School
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  • For correspondence: rosalind_segal@dfci.harvard.edu
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Abstract

The peripheral nervous system responds to a wide variety of sensory stimuli, a process that requires great neuronal diversity. These diverse peripheral sensory neurons are closely associated with glial cells that originate from the neural crest (NC). However, the molecular nature and origins of diversity among peripheral glia is not understood. Here we used single cell RNA sequencing to profile and compare developing and mature glia from somatosensory lumbar dorsal root ganglia (DRG) and auditory spiral ganglia (SG). We found that the glial precursors (GPs) differ in their transcriptional profile and prevalence in these two systems. Despite their unique features, somatosensory and auditory GPs undergo convergent differentiation to generate myelinating and non-myelinating Schwann cells that are molecularly uniform. By contrast, although satellite glia surround the neuronal cell bodies in both ganglia, we found that those in the SG express multiple myelination-associated genes, while DRG satellite cells express components that suppress myelination. Lastly, we identified a set of glial signature genes that are also expressed by placode-derived supporting cells, providing new insights into commonalities among glia across the nervous system. This comprehensive survey of gene expression in peripheral glia constitutes a valuable resource for understanding how glia acquire specialized functions and how their roles differ across sensory modalities.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted December 06, 2020.
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Diversity of developing peripheral glia revealed by single cell RNA sequencing
OE Tasdemir-Yilmaz, NR Druckenbrod, OO Olukoya, AR Yung, I Bastille, MF Pazyra-Murphy, A Sitko, EB Hale, S Vigneau, AA Gimelbrant, P Kharchenko, LV Goodrich, RA Segal
bioRxiv 2020.12.04.411975; doi: https://doi.org/10.1101/2020.12.04.411975
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Diversity of developing peripheral glia revealed by single cell RNA sequencing
OE Tasdemir-Yilmaz, NR Druckenbrod, OO Olukoya, AR Yung, I Bastille, MF Pazyra-Murphy, A Sitko, EB Hale, S Vigneau, AA Gimelbrant, P Kharchenko, LV Goodrich, RA Segal
bioRxiv 2020.12.04.411975; doi: https://doi.org/10.1101/2020.12.04.411975

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