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Inferring spikes from calcium imaging in dopamine neurons

Weston Fleming, Sean Jewell, Ben Engelhard, Daniela M. Witten, Ilana B. Witten
doi: https://doi.org/10.1101/2020.12.05.412965
Weston Fleming
1Princeton Neuroscience Institute, Princeton University, Princeton NJ 08544
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Sean Jewell
2Department of Statistics & Biostatistics, University of Washington
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Ben Engelhard
1Princeton Neuroscience Institute, Princeton University, Princeton NJ 08544
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Daniela M. Witten
2Department of Statistics & Biostatistics, University of Washington
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Ilana B. Witten
1Princeton Neuroscience Institute, Princeton University, Princeton NJ 08544
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  • For correspondence: iwitten@princeton.edu
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Abstract

Calcium imaging has led to discoveries about neural correlates of behavior in subcortical neurons, including dopamine (DA) neurons. However, spike inference methods have not been tested in most populations of subcortical neurons. To address this gap, we simultaneously performed calcium imaging and electrophysiology in DA neurons in brain slices, and applied a recently developed spike inference algorithm to the GCaMP fluorescence. This revealed that individual spikes can be inferred accurately in this population. Next, we inferred spikes in vivo from calcium imaging from these neurons during Pavlovian conditioning, as well as during navigation in virtual reality. In both cases, we quantitatively recapitulated previous in vivo electrophysiological observations. Our work provides a validated approach to infer spikes from calcium imaging in DA neurons, and implies that aspects of both tonic and phasic spike patterns can be recovered.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted December 06, 2020.
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Inferring spikes from calcium imaging in dopamine neurons
Weston Fleming, Sean Jewell, Ben Engelhard, Daniela M. Witten, Ilana B. Witten
bioRxiv 2020.12.05.412965; doi: https://doi.org/10.1101/2020.12.05.412965
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Inferring spikes from calcium imaging in dopamine neurons
Weston Fleming, Sean Jewell, Ben Engelhard, Daniela M. Witten, Ilana B. Witten
bioRxiv 2020.12.05.412965; doi: https://doi.org/10.1101/2020.12.05.412965

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