Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Robust integrated intracellular organization of the human iPS cell: where, how much, and how variable?

Matheus P. Viana, Jianxu Chen, Theo A. Knijnenburg, Ritvik Vasan, Calysta Yan, Joy E. Arakaki, Matte Bailey, Ben Berry, Antoine Borensztejn, Jackson M. Brown, Sara Carlson, Julie A. Cass, Basudev Chaudhuri, Kimberly R. Cordes Metzler, Mackenzie E. Coston, Zach J. Crabtree, Steve Davidson, Colette M. DeLizo, Shailja Dhaka, Stephanie Q. Dinh, Thao P. Do, Justin Domingus, Rory M. Donovan-Maiye, Tyler J. Foster, Christopher L. Frick, Griffin Fujioka, Margaret A. Fuqua, Jamie L. Gehring, Kaytlyn A. Gerbin, Tanya Grancharova, Benjamin W. Gregor, Lisa Harrylock, Amanda Haupt, Melissa C. Hendershott, Caroline Hookway, Alan R. Horwitz, Chris Hughes, Eric J. Isaac, Gregory R. Johnson, Brian Kim, Andrew N. Leonard, Winnie Leung, Jordan J. Lucas, Susan A. Ludmann, Blair M. Lyons, Haseeb Malik, Ryan McGregor, Gabe E. Medrash, Sean L. Meharry, Kevin Mitcham, Irina A. Mueller, Timothy L. Murphy-Stevens, Aditya Nath, Angelique M. Nelson, Luana Paleologu, T. Alexander Popiel, Megan M. Riel-Mehan, Brock Roberts, Lisa M. Schaefbauer, Magdalena Schwarzl, Jamie Sherman, Sylvain Slaton, M. Filip Sluzewski, Jacqueline E. Smith, Youngmee Sul, Madison J. Swain-Bowden, W. Joyce Tang, Derek J. Thirstrup, Daniel T. Toloudis, Andrew P. Tucker, Veronica Valencia, Winfried Wiegraebe, Thushara Wijeratna, Ruian Yang, Rebecca J. Zaunbrecher, Allen Institute for Cell Science, Graham T. Johnson, Ruwanthi N. Gunawardane, Nathalie Gaudreault, Julie A. Theriot, Susanne M. Rafelski
doi: https://doi.org/10.1101/2020.12.08.415562
Matheus P. Viana
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jianxu Chen
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Theo A. Knijnenburg
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ritvik Vasan
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Calysta Yan
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Joy E. Arakaki
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Matte Bailey
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ben Berry
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Antoine Borensztejn
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jackson M. Brown
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sara Carlson
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julie A. Cass
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Basudev Chaudhuri
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kimberly R. Cordes Metzler
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mackenzie E. Coston
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Zach J. Crabtree
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Steve Davidson
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Colette M. DeLizo
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Shailja Dhaka
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stephanie Q. Dinh
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thao P. Do
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Justin Domingus
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rory M. Donovan-Maiye
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tyler J. Foster
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Christopher L. Frick
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Griffin Fujioka
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Margaret A. Fuqua
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jamie L. Gehring
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kaytlyn A. Gerbin
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Tanya Grancharova
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Benjamin W. Gregor
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lisa Harrylock
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Amanda Haupt
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Melissa C. Hendershott
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Caroline Hookway
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alan R. Horwitz
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Chris Hughes
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eric J. Isaac
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gregory R. Johnson
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Brian Kim
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andrew N. Leonard
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Winnie Leung
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jordan J. Lucas
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Susan A. Ludmann
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Blair M. Lyons
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Haseeb Malik
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ryan McGregor
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gabe E. Medrash
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sean L. Meharry
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kevin Mitcham
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Irina A. Mueller
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Timothy L. Murphy-Stevens
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aditya Nath
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Angelique M. Nelson
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Luana Paleologu
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
T. Alexander Popiel
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Megan M. Riel-Mehan
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Brock Roberts
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Lisa M. Schaefbauer
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Magdalena Schwarzl
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jamie Sherman
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sylvain Slaton
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M. Filip Sluzewski
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jacqueline E. Smith
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Youngmee Sul
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Madison J. Swain-Bowden
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
W. Joyce Tang
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Derek J. Thirstrup
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniel T. Toloudis
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Andrew P. Tucker
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Veronica Valencia
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Winfried Wiegraebe
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thushara Wijeratna
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ruian Yang
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rebecca J. Zaunbrecher
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Graham T. Johnson
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ruwanthi N. Gunawardane
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nathalie Gaudreault
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julie A. Theriot
2Department of Biology and Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Susanne M. Rafelski
4Allen Institute for Cell Science, 615 Westlake Ave N, Seattle, WA, 98125, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: susanner@alleninstitute.org
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Summary

Despite the intimate link between cell organization and function, the principles underlying intracellular organization and the relation between organization, gene expression and phenotype are not well understood. We address this by creating a benchmark for mean cell organization and the natural range of cell-to-cell variation. This benchmark can be used for comparison to other normal or abnormal cell states. To do this, we developed a reproducible microscope imaging pipeline to generate a high quality dataset of 3D, high-resolution images of over 200,000 live cells from 25 isogenic human induced pluripotent stem cell (hiPSC) lines from the Allen Cell Collection. Each line contains one fluorescently tagged protein, created via endogenous CRISPR/Cas9 gene editing, representing a key cellular structure or organelle. We used these images to develop a new multi-part generalizable analysis approach of the locations, amounts, and variation of the 25 cellular structures. Taking an integrated approach, we found that both the extent to which a structure’s individual location varied (“stereotypy”) and the extent to which the structure localized relative to all the other cellular structures (“concordance”) were robust to a wide range of cell shape variation, from flatter to taller, smaller to larger, or less to more polarized cells. We also found that these cellular structures varied greatly in how their volumes scaled with cell and nuclear size. These analyses create a data-driven set of quantitative rules for the locations, amounts, and variation of 25 cellular structures within the hiPSC as a normal baseline for cell organization.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted December 10, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Robust integrated intracellular organization of the human iPS cell: where, how much, and how variable?
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Robust integrated intracellular organization of the human iPS cell: where, how much, and how variable?
Matheus P. Viana, Jianxu Chen, Theo A. Knijnenburg, Ritvik Vasan, Calysta Yan, Joy E. Arakaki, Matte Bailey, Ben Berry, Antoine Borensztejn, Jackson M. Brown, Sara Carlson, Julie A. Cass, Basudev Chaudhuri, Kimberly R. Cordes Metzler, Mackenzie E. Coston, Zach J. Crabtree, Steve Davidson, Colette M. DeLizo, Shailja Dhaka, Stephanie Q. Dinh, Thao P. Do, Justin Domingus, Rory M. Donovan-Maiye, Tyler J. Foster, Christopher L. Frick, Griffin Fujioka, Margaret A. Fuqua, Jamie L. Gehring, Kaytlyn A. Gerbin, Tanya Grancharova, Benjamin W. Gregor, Lisa Harrylock, Amanda Haupt, Melissa C. Hendershott, Caroline Hookway, Alan R. Horwitz, Chris Hughes, Eric J. Isaac, Gregory R. Johnson, Brian Kim, Andrew N. Leonard, Winnie Leung, Jordan J. Lucas, Susan A. Ludmann, Blair M. Lyons, Haseeb Malik, Ryan McGregor, Gabe E. Medrash, Sean L. Meharry, Kevin Mitcham, Irina A. Mueller, Timothy L. Murphy-Stevens, Aditya Nath, Angelique M. Nelson, Luana Paleologu, T. Alexander Popiel, Megan M. Riel-Mehan, Brock Roberts, Lisa M. Schaefbauer, Magdalena Schwarzl, Jamie Sherman, Sylvain Slaton, M. Filip Sluzewski, Jacqueline E. Smith, Youngmee Sul, Madison J. Swain-Bowden, W. Joyce Tang, Derek J. Thirstrup, Daniel T. Toloudis, Andrew P. Tucker, Veronica Valencia, Winfried Wiegraebe, Thushara Wijeratna, Ruian Yang, Rebecca J. Zaunbrecher, Allen Institute for Cell Science, Graham T. Johnson, Ruwanthi N. Gunawardane, Nathalie Gaudreault, Julie A. Theriot, Susanne M. Rafelski
bioRxiv 2020.12.08.415562; doi: https://doi.org/10.1101/2020.12.08.415562
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Robust integrated intracellular organization of the human iPS cell: where, how much, and how variable?
Matheus P. Viana, Jianxu Chen, Theo A. Knijnenburg, Ritvik Vasan, Calysta Yan, Joy E. Arakaki, Matte Bailey, Ben Berry, Antoine Borensztejn, Jackson M. Brown, Sara Carlson, Julie A. Cass, Basudev Chaudhuri, Kimberly R. Cordes Metzler, Mackenzie E. Coston, Zach J. Crabtree, Steve Davidson, Colette M. DeLizo, Shailja Dhaka, Stephanie Q. Dinh, Thao P. Do, Justin Domingus, Rory M. Donovan-Maiye, Tyler J. Foster, Christopher L. Frick, Griffin Fujioka, Margaret A. Fuqua, Jamie L. Gehring, Kaytlyn A. Gerbin, Tanya Grancharova, Benjamin W. Gregor, Lisa Harrylock, Amanda Haupt, Melissa C. Hendershott, Caroline Hookway, Alan R. Horwitz, Chris Hughes, Eric J. Isaac, Gregory R. Johnson, Brian Kim, Andrew N. Leonard, Winnie Leung, Jordan J. Lucas, Susan A. Ludmann, Blair M. Lyons, Haseeb Malik, Ryan McGregor, Gabe E. Medrash, Sean L. Meharry, Kevin Mitcham, Irina A. Mueller, Timothy L. Murphy-Stevens, Aditya Nath, Angelique M. Nelson, Luana Paleologu, T. Alexander Popiel, Megan M. Riel-Mehan, Brock Roberts, Lisa M. Schaefbauer, Magdalena Schwarzl, Jamie Sherman, Sylvain Slaton, M. Filip Sluzewski, Jacqueline E. Smith, Youngmee Sul, Madison J. Swain-Bowden, W. Joyce Tang, Derek J. Thirstrup, Daniel T. Toloudis, Andrew P. Tucker, Veronica Valencia, Winfried Wiegraebe, Thushara Wijeratna, Ruian Yang, Rebecca J. Zaunbrecher, Allen Institute for Cell Science, Graham T. Johnson, Ruwanthi N. Gunawardane, Nathalie Gaudreault, Julie A. Theriot, Susanne M. Rafelski
bioRxiv 2020.12.08.415562; doi: https://doi.org/10.1101/2020.12.08.415562

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4119)
  • Biochemistry (8828)
  • Bioengineering (6531)
  • Bioinformatics (23484)
  • Biophysics (11804)
  • Cancer Biology (9222)
  • Cell Biology (13336)
  • Clinical Trials (138)
  • Developmental Biology (7442)
  • Ecology (11424)
  • Epidemiology (2066)
  • Evolutionary Biology (15173)
  • Genetics (10452)
  • Genomics (14056)
  • Immunology (9187)
  • Microbiology (22198)
  • Molecular Biology (8823)
  • Neuroscience (47621)
  • Paleontology (351)
  • Pathology (1431)
  • Pharmacology and Toxicology (2493)
  • Physiology (3736)
  • Plant Biology (8090)
  • Scientific Communication and Education (1438)
  • Synthetic Biology (2224)
  • Systems Biology (6042)
  • Zoology (1254)