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Cryo-EM structures of outer-arm dynein array bound to microtubule doublet reveal a mechanism for motor coordination

Qinhui Rao, Yue Wang, Pengxin Chai, Yin-Wei Kuo, Long Han, Renbin Yang, Yuchen Yang, Jonathon Howard, View ORCID ProfileKai Zhang
doi: https://doi.org/10.1101/2020.12.08.415687
Qinhui Rao
1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06511, USA
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Yue Wang
1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06511, USA
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Pengxin Chai
1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06511, USA
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Yin-Wei Kuo
1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06511, USA
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Long Han
1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06511, USA
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Renbin Yang
1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06511, USA
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Yuchen Yang
1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06511, USA
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Jonathon Howard
1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06511, USA
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Kai Zhang
1Department of Molecular Biophysics and Biochemistry, Yale University, New Haven, CT, 06511, USA
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  • ORCID record for Kai Zhang
  • For correspondence: jack.zhang@yale.edu
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Abstract

Thousands of outer-arm dyneins (OADs) are arrayed in the axoneme to drive a rhythmic ciliary beat. Using electron microscopy, we determined the structure of OAD array bound to microtubule doublets (MTDs) in near-atomic details and illuminate how OADs coordinate with each other to move one step forward. OAD prefers a specific pattern of MTD protofilaments for its distinct microtubule-binding domains. Upon MTD binding, free OADs are induced to adopt a stable parallel conformation, primed for array formation. Extensive tail-to-head (TTH) interactions between OADs are observed, which need to be broken for ATP turnover by the dynein motor. ATP-hydrolysis in turn relaxes the TTH interfaces to sequentially effectuate free nucleotide cycle of downstream OADs. These findings lead to a model for how conformational changes of OADs produce coordinated actions.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted December 08, 2020.
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Cryo-EM structures of outer-arm dynein array bound to microtubule doublet reveal a mechanism for motor coordination
Qinhui Rao, Yue Wang, Pengxin Chai, Yin-Wei Kuo, Long Han, Renbin Yang, Yuchen Yang, Jonathon Howard, Kai Zhang
bioRxiv 2020.12.08.415687; doi: https://doi.org/10.1101/2020.12.08.415687
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Cryo-EM structures of outer-arm dynein array bound to microtubule doublet reveal a mechanism for motor coordination
Qinhui Rao, Yue Wang, Pengxin Chai, Yin-Wei Kuo, Long Han, Renbin Yang, Yuchen Yang, Jonathon Howard, Kai Zhang
bioRxiv 2020.12.08.415687; doi: https://doi.org/10.1101/2020.12.08.415687

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