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Multiple clones of colistin-resistant Salmonella enterica carrying mcr-1 plasmids in meat products and patients in Northern Thailand

View ORCID ProfilePrapas Patchanee, View ORCID ProfileNipa Chokesajjawatee, Pannita Santiyanont, View ORCID ProfilePhongsakorn Chuammitri, Manu Deeudom, View ORCID ProfileWilliam Monteith, View ORCID ProfileSamuel K. Sheppard, View ORCID ProfileBen Pascoe, Teerarat Prasertsee
doi: https://doi.org/10.1101/2020.12.08.415869
Prapas Patchanee
aIntegrative Research Center for Veterinary Preventive Medicine, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand
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Nipa Chokesajjawatee
bNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand
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Pannita Santiyanont
bNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathum Thani, 12120, Thailand
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Phongsakorn Chuammitri
cDepartment of Veterinary Biosciences and Public Health, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand
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Manu Deeudom
dDepartment of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand
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William Monteith
eThe Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, BA2 7BA, United Kingdom
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Samuel K. Sheppard
eThe Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, BA2 7BA, United Kingdom
fDivision of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand
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Ben Pascoe
aIntegrative Research Center for Veterinary Preventive Medicine, Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand
eThe Milner Centre for Evolution, Department of Biology and Biochemistry, University of Bath, BA2 7BA, United Kingdom
fDivision of Clinical Microbiology, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand
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  • For correspondence: b.pascoe@bath.ac.uk teerarat.p@psu.ac.th
Teerarat Prasertsee
gFaculty of Veterinary Science, Prince of Songkla University, Songkhla, 90110, Thailand
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  • For correspondence: b.pascoe@bath.ac.uk teerarat.p@psu.ac.th
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Abstract

Salmonella spp. is an important foodborne pathogen associated with consumption of contaminated food, especially livestock products. Antimicrobial resistance (AMR) in Salmonella has been reported globally and increasing AMR in food production is a major public health issue worldwide. The objective of this study was to describe the genetic relatedness among Salmonella enterica isolates, which displayed identical DNA fingerprint profiles. Ten S. enterica isolates were selected from meat and human cases with an identical rep-PCR profile of serovars Rissen (n=4), Weltevreden (n=4), and Stanley (n=2). We used long-read whole genome sequencing (WGS) on the MinION sequencing platform to type isolates and investigate in silico the presence of specific AMR genes. Antimicrobial susceptibility testing was tested by disk diffusion and gradient diffusion method to corroborate the AMR phenotype. Multidrug resistance and resistance to more than one antimicrobial agent were observed in eight and nine isolates, respectively. Resistance to colistin with an accompanying mcr-1 gene was observed among the Salmonella isolates. The analysis of core genome and whole genome MLST revealed that the Salmonella from meat and human salmonellosis were closely genetic related. Hence, it could be concluded that meat is one of the important sources for Salmonella infection in human.

Highlights

  • Colistin resistance detected in 2 clones from 2 different Salmonella enterica serovars (Rissen and Weltevreden) with accompanying plasmid-borne mcr-1 gene from the food production chain and human clinical salmonellosis.

  • High prevalence of multidrug resistant isolates and resistance to more than one antimicrobial agent.

  • MinION has potential for mobile, rapid and accurate application in veterinary genomic epidemiology studies.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • https://www.ncbi.nlm.nih.gov/bioproject/PRJNA606456

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Multiple clones of colistin-resistant Salmonella enterica carrying mcr-1 plasmids in meat products and patients in Northern Thailand
Prapas Patchanee, Nipa Chokesajjawatee, Pannita Santiyanont, Phongsakorn Chuammitri, Manu Deeudom, William Monteith, Samuel K. Sheppard, Ben Pascoe, Teerarat Prasertsee
bioRxiv 2020.12.08.415869; doi: https://doi.org/10.1101/2020.12.08.415869
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Multiple clones of colistin-resistant Salmonella enterica carrying mcr-1 plasmids in meat products and patients in Northern Thailand
Prapas Patchanee, Nipa Chokesajjawatee, Pannita Santiyanont, Phongsakorn Chuammitri, Manu Deeudom, William Monteith, Samuel K. Sheppard, Ben Pascoe, Teerarat Prasertsee
bioRxiv 2020.12.08.415869; doi: https://doi.org/10.1101/2020.12.08.415869

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