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A vacuolar hexose transport is required for xylem development in the inflorescence stem of Arabidopsis

Emilie Aubry, Beate Hoffmann, Françoise Vilaine, View ORCID ProfileFrançoise Gilard, Patrick A.W. Klemens, Florence Guérard, View ORCID ProfileBertrand Gakière, View ORCID ProfileH. Ekkehard Neuhaus, View ORCID ProfileCatherine Bellini, View ORCID ProfileSylvie Dinant, View ORCID ProfileRozenn Le Hir
doi: https://doi.org/10.1101/2020.12.09.417345
Emilie Aubry
1Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, France
2Ecole Doctorale 567 Sciences du Végétal, Univ Paris-Sud, Univ Paris-Saclay, bat 360, 91405 Orsay Cedex, France
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Beate Hoffmann
1Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, France
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Françoise Vilaine
1Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, France
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Françoise Gilard
3Plateforme Métabolisme-Métabolome, Institute of Plant Sciences Paris-Saclay IPS2, CNRS, INRAE, Univ Paris Sud, Univ Evry, Univ Paris-Diderot, Sorbonne Paris-Cité, Université Paris-Saclay, Bâtiment 360, Rue de Noetzlin, 91192 Gif sur Yvette, France
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Patrick A.W. Klemens
4Universität Kaiserslautern, Pflanzenphysiologie, Postfach 3049, D-67653 Kaiserslautern, Germany
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Florence Guérard
3Plateforme Métabolisme-Métabolome, Institute of Plant Sciences Paris-Saclay IPS2, CNRS, INRAE, Univ Paris Sud, Univ Evry, Univ Paris-Diderot, Sorbonne Paris-Cité, Université Paris-Saclay, Bâtiment 360, Rue de Noetzlin, 91192 Gif sur Yvette, France
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Bertrand Gakière
3Plateforme Métabolisme-Métabolome, Institute of Plant Sciences Paris-Saclay IPS2, CNRS, INRAE, Univ Paris Sud, Univ Evry, Univ Paris-Diderot, Sorbonne Paris-Cité, Université Paris-Saclay, Bâtiment 360, Rue de Noetzlin, 91192 Gif sur Yvette, France
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H. Ekkehard Neuhaus
4Universität Kaiserslautern, Pflanzenphysiologie, Postfach 3049, D-67653 Kaiserslautern, Germany
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Catherine Bellini
1Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, France
5Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 90187 Umeå, Sweden
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Sylvie Dinant
1Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, France
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Rozenn Le Hir
1Institut Jean-Pierre Bourgin, INRAE, AgroParisTech, Université Paris-Saclay, 78000 Versailles, France
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  • For correspondence: rozenn.le-hir@inrae.fr
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ABSTRACT

In higher plants, the development of the vascular system is controlled by a complex network of transcription factors. However, how nutrient availability in the vascular cells affects their development remains to be addressed. At the cellular level, cytosolic sugar availability is regulated mainly by sugar exchanges at the tonoplast through active and/or facilitated transport. In Arabidopsis thaliana, among the tonoplastic transporters, SWEET16 and SWEET17 have been previously localized in the vascular system. Here, using a reverse genetic approach, we propose that sugar exchanges at the tonoplast, mediated by SWEET16, are important for xylem cell division as revealed in particular by the decreased number of xylem cells in the swt16 mutant and the expression of SWEET16 at the procambium-xylem boundary. In addition, we demonstrate that transport of hexoses mediated by SWEET16 and/or SWEET17 is required to sustain the formation of the xylem secondary cell wall. This result is in line with a defect in the xylem cell wall composition as measured by FTIR in the swt16swt17 double mutant and by upregulation of several genes involved in secondary cell wall synthesis. Our work therefore supports a model in which xylem development is partially dependent on the exchange of hexoses at the tonoplast of xylem-forming cells.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • One-sentence summary: A control of cytosolic sugar availability, mediated by SWEET16 and SWEET17, is required to sustain xylem development and secondary cell wall formation.

  • Funding information: This work has benefited from the support of IJPB’s Plant Observatory technological platforms and from a French State grant (Saclay Plant Sciences, reference ANR-17-EUR-0007, EUR SPS-GSR) managed by the French National Research Agency under an Investments for the Future program (reference ANR-11-IDEX-0003-02) through PhD funding to E.A.

  • Manuscript title updated

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 12, 2021.
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A vacuolar hexose transport is required for xylem development in the inflorescence stem of Arabidopsis
Emilie Aubry, Beate Hoffmann, Françoise Vilaine, Françoise Gilard, Patrick A.W. Klemens, Florence Guérard, Bertrand Gakière, H. Ekkehard Neuhaus, Catherine Bellini, Sylvie Dinant, Rozenn Le Hir
bioRxiv 2020.12.09.417345; doi: https://doi.org/10.1101/2020.12.09.417345
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A vacuolar hexose transport is required for xylem development in the inflorescence stem of Arabidopsis
Emilie Aubry, Beate Hoffmann, Françoise Vilaine, Françoise Gilard, Patrick A.W. Klemens, Florence Guérard, Bertrand Gakière, H. Ekkehard Neuhaus, Catherine Bellini, Sylvie Dinant, Rozenn Le Hir
bioRxiv 2020.12.09.417345; doi: https://doi.org/10.1101/2020.12.09.417345

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