Abstract
The inflamed rheumatic joint is a highly heterogeneous and complex tissue with dynamic recruitment and expansion of multiple cell types that interact in multifaceted ways within a localized area. Rheumatoid arthritis synovium has primarily been studied either by immunostaining or by molecular profiling after tissue homogenization. Here, we use Spatial Transcriptomics to study local cellular interactions at the site of chronic synovial inflammation. We report comprehensive spatial RNA-seq data coupled to quantitative and cell type-specific chemokine-driven dynamics at and around organized structures of infiltrating leukocyte cells in the synovium.
Competing Interest Statement
S.V and P.L.S. are authors on patents applied for by Spatial Transcriptomics AB (10X Genomics Inc) covering the technology. P.L.S. and K.C. are scientific consultants to 10x Genomics Inc. P.K.S is on the SAB or BOD of Rarecyte Inc, Applied Biomath LLC, Glencoe Software Inc. and Nanostring Inc.; none of these relationships are directly related to the topic of this study. A.R. is a founder and equity holder of Celsius Therapeutics, an equity holder in Immunitas Therapeutics and until August 31, 2020 was an SAB member of Syros Pharmaceuticals, Neogene Therapeutics, Asimov and ThermoFisher Scientific. From August 1, 2020, A.R. is an employee of Genentech, a member of the Roche Group. S.V. and A.R. are co-inventors on PCT/US2020/015481 relating to this work.
