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Glycomic analysis reveals a conserved response to bacterial sepsis induced by different bacterial pathogens

Daniel W. Heindel, Peter V. Aziz, Shuhui Chen, Jamey D. Marth, View ORCID ProfileLara K. Mahal
doi: https://doi.org/10.1101/2020.12.11.421610
Daniel W. Heindel
1Biomedical Research Institute, Department of Chemistry, New York University, NY
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Peter V. Aziz
2Center for Nanomedicine, SBP Medical Discovery Institute, Department of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara
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Shuhui Chen
1Biomedical Research Institute, Department of Chemistry, New York University, NY
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Jamey D. Marth
2Center for Nanomedicine, SBP Medical Discovery Institute, Department of Molecular, Cellular, and Developmental Biology, University of California Santa Barbara
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Lara K. Mahal
1Biomedical Research Institute, Department of Chemistry, New York University, NY
3Department of Chemistry, University of Alberta, Edmonton, AB, CANADA
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  • ORCID record for Lara K. Mahal
  • For correspondence: lkmahal@ualberta.ca
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Abstract

Sepsis is an extreme inflammatory response to infection (bacterial, viral, fungal) that occurs in the bloodstream and causes damage throughout the body. Currently, there are few diagnostic biomarkers of sepsis and new effective treatments have not been developed. There is a clear need to study the molecular underpinnings of this disease. Glycosylation is known to play a role in immunity and inflammation, but the role of glycans in sepsis is not well defined. Herein, we profiled the serum glycomes of experimental mouse sepsis models to identify changes induced by 4 different clinical bacterial pathogens (Gram-positive: Streptococcus pneumoniae, Staphylococcus aureus, Gram-negative: Escherichia coli and Salmonella Typhimurium) using our lectin microarray technology. We observed global shifts in the blood sera glycome that were conserved across all four species, regardless of whether they were Gram positive or negative. Bisecting GlcNAc was decreased upon sepsis and a strong increase in core 1/3 O-glycans was observed. Lectin blot analysis revealed a high molecular weight protein induced in sepsis by all four bacteria as the major cause of the core 1/3 O-glycan shift. While the identity of this protein remains to be elucidated, its presence indicates a common feature of bacterial sepsis associated with this glycomic signature.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license.
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Posted December 11, 2020.
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Glycomic analysis reveals a conserved response to bacterial sepsis induced by different bacterial pathogens
Daniel W. Heindel, Peter V. Aziz, Shuhui Chen, Jamey D. Marth, Lara K. Mahal
bioRxiv 2020.12.11.421610; doi: https://doi.org/10.1101/2020.12.11.421610
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Glycomic analysis reveals a conserved response to bacterial sepsis induced by different bacterial pathogens
Daniel W. Heindel, Peter V. Aziz, Shuhui Chen, Jamey D. Marth, Lara K. Mahal
bioRxiv 2020.12.11.421610; doi: https://doi.org/10.1101/2020.12.11.421610

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