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A low-autofluorescence, transparent resin for multiphoton 3D printing

George Flamourakis, Antonis Kordas, Georgios D. Barmparis, Anthi Ranella, View ORCID ProfileMaria Farsari
doi: https://doi.org/10.1101/2020.12.15.422922
George Flamourakis
1Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas (FORTH/IESL) GR-70013 Heraklion, Crete, Greece
2Department of Materials Science and Technology, University of Crete, GR-7001 Heraklion, Crete, Greece
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Antonis Kordas
1Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas (FORTH/IESL) GR-70013 Heraklion, Crete, Greece
2Department of Materials Science and Technology, University of Crete, GR-7001 Heraklion, Crete, Greece
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Georgios D. Barmparis
3Institute of Theoretical and Computational Physics and Department of Physics, University of Crete, P.O. Box 2208, 71003 Heraklion, Greece
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Anthi Ranella
1Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas (FORTH/IESL) GR-70013 Heraklion, Crete, Greece
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  • For correspondence: mfarsari@iesl.forth.gr ranthi@iesl.forth.gr
Maria Farsari
1Institute of Electronic Structure and Laser, Foundation for Research and Technology-Hellas (FORTH/IESL) GR-70013 Heraklion, Crete, Greece
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  • ORCID record for Maria Farsari
  • For correspondence: mfarsari@iesl.forth.gr ranthi@iesl.forth.gr
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Abstract

Multiphoton lithography allows the high resolution, free-form 3D printing of structures such as micro-optical elements and 3D scaffolds for Tissue Engineering. A major obstacle in its application in these fields is material and structure autofluorescence. Existing photoresists promise near zero fluorescent in expense of poor mechanical properties, and low printing efficiency. Sudan Black B is a molecular quencher used as a dye for biological studies and as means of decreasing the autofluorescence of polymers. In our study we report the use of Sudan Black B as both a photoinitiator and as a post-fabrication treatment step, using the zirconium silicate SZ2080™ for the development of a non-fluorescent composite. We use this material for the 3D printing of micro-optical elements, and meso-scale scaffolds for Mesenchymal Stem Cell cultures. Our results show the hybrid, made photosensitive with Sudan Black B, can be used for the fabrication of high resolution, highly transparent, autofluorescence-free microstructures.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted December 16, 2020.
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A low-autofluorescence, transparent resin for multiphoton 3D printing
George Flamourakis, Antonis Kordas, Georgios D. Barmparis, Anthi Ranella, Maria Farsari
bioRxiv 2020.12.15.422922; doi: https://doi.org/10.1101/2020.12.15.422922
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A low-autofluorescence, transparent resin for multiphoton 3D printing
George Flamourakis, Antonis Kordas, Georgios D. Barmparis, Anthi Ranella, Maria Farsari
bioRxiv 2020.12.15.422922; doi: https://doi.org/10.1101/2020.12.15.422922

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