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Whole plastid genome-based phylogenomics supports an inner placement of the O. insectifera group rather than a basal position in the rapidly diversifying Ophrys genus (Orchidaceae)

View ORCID ProfileJoris A. M. Bertrand, View ORCID ProfileAnaïs Gibert, Christel Llauro, View ORCID ProfileOlivier Panaud
doi: https://doi.org/10.1101/2020.12.16.423003
Joris A. M. Bertrand
aLaboratoire Génome & Développement des Plantes (UMR 5096 UPVD/CNRS), Université de Perpignan Via Domitia, Perpignan, France
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  • For correspondence: joris.bertrand@univ-perp.fr
Anaïs Gibert
bPSL Université Paris, EPHE-UPVD-CNRS, USR 3278 CRIOBE, Université de Perpignan Via Domitia, France
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Christel Llauro
aLaboratoire Génome & Développement des Plantes (UMR 5096 UPVD/CNRS), Université de Perpignan Via Domitia, Perpignan, France
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Olivier Panaud
aLaboratoire Génome & Développement des Plantes (UMR 5096 UPVD/CNRS), Université de Perpignan Via Domitia, Perpignan, France
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Abstract

Some lineages of the Orchid genus Ophrys exhibit among the highest diversification rates reported so far. As a consequence of a such intense and rapid evolution, the systematics and the taxonomy of this genus remains unclear. A hybrid assembly approach based-on long- and short-read genomic data allowed us to outperform classical methods to successfully assemble whole plastid genomes for two new Ophrys species: O. aymoninii and O. lutea. Along with three other previously Ophrys plastid genome sequences, we then reconstructed the first whole plastome-based molecular phylogeny including representatives of the three mains recognized Ophrys lineages. Our results support the placement of the O. insectifera clade as sister group of ‘non-basal Ophrys’ rather than a basal position. Our findings corroborate recent results obtained from genomic data (RAD-seq and transcriptomes) but contrast with previous ones. These results therefore confirm that molecular phylogenetic hypotheses based on a limited number of loci (e.g. nrITS, matK, rbcL) may have provided a biased picture of phylogenetic relationships within Ophrys and possibly other plant taxa.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Joris Bertrand conducted fieldwork, analyzed the data and wrote the manuscript.

  • Anaïs Gibert contributed to fieldwork and manuscript writing.

  • Christel Llauro generated the long-read data set.

  • Olivier Panaud contributed to research funding and manuscript writing.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
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Posted February 16, 2021.
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Whole plastid genome-based phylogenomics supports an inner placement of the O. insectifera group rather than a basal position in the rapidly diversifying Ophrys genus (Orchidaceae)
Joris A. M. Bertrand, Anaïs Gibert, Christel Llauro, Olivier Panaud
bioRxiv 2020.12.16.423003; doi: https://doi.org/10.1101/2020.12.16.423003
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Whole plastid genome-based phylogenomics supports an inner placement of the O. insectifera group rather than a basal position in the rapidly diversifying Ophrys genus (Orchidaceae)
Joris A. M. Bertrand, Anaïs Gibert, Christel Llauro, Olivier Panaud
bioRxiv 2020.12.16.423003; doi: https://doi.org/10.1101/2020.12.16.423003

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