Abstract
Recent studies proposed that active sites induce long-range evolutionary constraints in enzymes. The physical origin of such long-range evolutionary coupling is unknown. Here, I study physical and evolutionary couplings in a diverse data set of monomeric enzymes, using a biophysical model of enzyme evolution. I show that evolutionary coupling is not universally long-range, but range varies widely among enzymes, from 2Å to 20Å. This cannot be explained by variations of physical coupling, which is short-range for all enzymes. Rather, the variation of evolutionary coupling range among enzymes is determined by a huge variation among them of functional selection pressure.
Competing Interest Statement
The authors have declared no competing interest.
Footnotes
Major revision.
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