ABSTRACT
Leaf number and leaf emergence rate are phenotypes of interest to plant breeders, plant geneticists, and crop modelers. Counting the leaves of an individual plant is straightforward even for an untrained individual, but manually tracking changes in leaf numbers for hundreds of individuals across multiple time points is logistically challenging. This study generated a dataset including over 150,000 maize and sorghum images for leaf counting projects. A subset of 17,783 images also includes annotations of the positions of individual leaf tips. With these annotated images, we evaluate two deep learning-based approaches for automated leaf counting: the first based on counting-by-regression from whole image analysis and a second based on counting-bydetection. Both approaches can achieve RMSE (root of mean square error) smaller than one leaf, only moderately inferior to the RMSE between human annotators of between 0.57 and 0.73 leaves. The counting-by-regression approach based on CNNs (convolutional neural networks) exhibited lower accuracy and increased bias for plants with extreme leave numbers which are underrepresented in this dataset. The counting-by-detection approach based on Faster R-CNNs object detection models achieve near human performance for plants where all leaf tips are visible. The annotated image data and model performance metrics generated as part of this study provide large scale resources for comparison and the improvement of algorithms for leaf counting from image data in grain crops.
Competing Interest Statement
The authors have declared no competing interest.
