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In-depth characterization of denitrifier communities across different soil ecosystems in the tundra

View ORCID ProfileIgor S. Pessi, View ORCID ProfileSirja Viitamäki, View ORCID ProfileAnna-Maria Virkkala, View ORCID ProfileEeva Eronen-Rasimus, View ORCID ProfileTom O. Delmont, View ORCID ProfileMaija E. Marushchak, View ORCID ProfileMiska Luoto, View ORCID ProfileJenni Hultman
doi: https://doi.org/10.1101/2020.12.21.419267
Igor S. Pessi
1Department of Microbiology, University of Helsinki, Helsinki, Finland
2Helsinki Institute of Sustainability Science (HELSUS), Helsinki, Finland
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Sirja Viitamäki
1Department of Microbiology, University of Helsinki, Helsinki, Finland
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Anna-Maria Virkkala
3Department of Geosciences and Geography, University of Helsinki, Helsinki, Finland
4Woodwell Climate Research Center, Falmouth, MA, USA
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Eeva Eronen-Rasimus
1Department of Microbiology, University of Helsinki, Helsinki, Finland
5Marine Research Centre, Finnish Environment Institute (SYKE), Helsinki, Finland
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Tom O. Delmont
6Department of Bioinformatics, Genoscope, Paris, France
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Maija E. Marushchak
7Department of Biological and Environmental Science, University of Jyväskylä, Jyväskylä, Finland
8Department of Environmental and Biological Sciences, University of Eastern Finland, Kuopio, Finland
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Miska Luoto
3Department of Geosciences and Geography, University of Helsinki, Helsinki, Finland
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Jenni Hultman
1Department of Microbiology, University of Helsinki, Helsinki, Finland
2Helsinki Institute of Sustainability Science (HELSUS), Helsinki, Finland
9Natural Resources Institute Finland (Luke), Helsinki, Finland
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  • For correspondence: jenni.hultman@helsinki.fi
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Abstract

Background In contrast to earlier assumptions, there is now mounting evidence for the role of tundra soils as important sources of the greenhouse gas nitrous oxide (N2O). However, the microorganisms involved in the cycling of N2O in this system remain largely uncharacterized. Since tundra soils are variable sources and sinks of N2O, we aimed at investigating differences in community structure across different soil ecosystems in the tundra.

Results We analysed 1.4 Tb of metagenomic data from soils in northern Finland covering a range of ecosystems from dry upland soils to water-logged fens and obtained 796 manually binned and curated metagenome-assembled genomes (MAGs). We then searched for MAGs harbouring genes involved in denitrification, an important process driving N2O emissions. Communities of potential denitrifiers were dominated by microorganisms with truncated denitrification pathways (i.e., lacking one or more denitrification genes) and differed across soil ecosystems. Upland soils showed a strong N2O sink potential and were dominated by members of the Alphaproteobacteria such as Bradyrhizobium and Reyranella. Fens, which had in general net-zero N2O fluxes, had a high abundance of poorly characterized taxa affiliated with the Chloroflexota lineage Ellin6529 and the Acidobacteriota subdivision Gp23.

Conclusions By coupling an in-depth characterization of microbial communities with in situ measurements of N2O fluxes, our results suggest that the observed spatial patterns of N2O fluxes in the tundra are related to differences in the composition of denitrifier communities.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted April 01, 2022.
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In-depth characterization of denitrifier communities across different soil ecosystems in the tundra
Igor S. Pessi, Sirja Viitamäki, Anna-Maria Virkkala, Eeva Eronen-Rasimus, Tom O. Delmont, Maija E. Marushchak, Miska Luoto, Jenni Hultman
bioRxiv 2020.12.21.419267; doi: https://doi.org/10.1101/2020.12.21.419267
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In-depth characterization of denitrifier communities across different soil ecosystems in the tundra
Igor S. Pessi, Sirja Viitamäki, Anna-Maria Virkkala, Eeva Eronen-Rasimus, Tom O. Delmont, Maija E. Marushchak, Miska Luoto, Jenni Hultman
bioRxiv 2020.12.21.419267; doi: https://doi.org/10.1101/2020.12.21.419267

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