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Single-cell Individual Complete mtDNA Sequencing Uncovers Hidden Mitochondrial Heterogeneity in Human and Mouse Oocytes

Chongwei Bi, Lin Wang, Yong Fan, Gerardo Ramos-Mandujano, Baolei Yuan, Xuan Zhou, Jincheng Wang, Yanjiao Shao, Pu-Yao Zhang, Yanyi Huang, Yang Yu, Juan Carlos Izpisua Belmonte, View ORCID ProfileMo Li
doi: https://doi.org/10.1101/2020.12.28.424537
Chongwei Bi
1Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
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Lin Wang
1Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
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Yong Fan
2Department of Obstetrics and Gynecology, Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, 510150 Guangzhou, China
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Gerardo Ramos-Mandujano
1Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
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Baolei Yuan
1Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
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Xuan Zhou
1Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
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Jincheng Wang
3Beijing Advanced Innovation Center for Genomics (ICG), Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, College of Chemistry, College of Engineering, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
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Yanjiao Shao
4Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA
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Pu-Yao Zhang
5Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
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Yanyi Huang
3Beijing Advanced Innovation Center for Genomics (ICG), Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, College of Chemistry, College of Engineering, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
6Institute for Cell Analysis, Shenzhen Bay Laboratory, Shenzhen, China
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Yang Yu
5Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
7Stem Cell Research Center, Peking University Third Hospital, Beijing 100191, China
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  • For correspondence: yuyang5012@hotmail.com Belmonte@salk.edu mo.li@kaust.edu.sa
Juan Carlos Izpisua Belmonte
4Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA
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  • For correspondence: yuyang5012@hotmail.com Belmonte@salk.edu mo.li@kaust.edu.sa
Mo Li
1Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
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  • ORCID record for Mo Li
  • For correspondence: yuyang5012@hotmail.com Belmonte@salk.edu mo.li@kaust.edu.sa
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Article Information

doi 
https://doi.org/10.1101/2020.12.28.424537
History 
  • December 28, 2020.
Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.

Author Information

  1. Chongwei Bi1,9,
  2. Lin Wang1,8,9,
  3. Yong Fan2,9,
  4. Gerardo Ramos-Mandujano1,
  5. Baolei Yuan1,
  6. Xuan Zhou1,
  7. Jincheng Wang3,
  8. Yanjiao Shao4,
  9. Pu-Yao Zhang5,
  10. Yanyi Huang3,6,
  11. Yang Yu5,7,*,
  12. Juan Carlos Izpisua Belmonte4,* and
  13. Mo Li1,*
  1. 1Biological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
  2. 2Department of Obstetrics and Gynecology, Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, 510150 Guangzhou, China
  3. 3Beijing Advanced Innovation Center for Genomics (ICG), Biomedical Pioneering Innovation Center (BIOPIC), School of Life Sciences, College of Chemistry, College of Engineering, Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, China
  4. 4Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, CA, 92037, USA
  5. 5Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing 100191, China
  6. 6Institute for Cell Analysis, Shenzhen Bay Laboratory, Shenzhen, China
  7. 7Stem Cell Research Center, Peking University Third Hospital, Beijing 100191, China
  1. ↵*Correspondence: yuyang5012{at}hotmail.com (Y. Y.), Belmonte{at}salk.edu (J.C.I.B.), mo.li{at}kaust.edu.sa (M.L.)
  1. ↵9 these authors contributed equally

  • ↵8 Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, China

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Posted December 28, 2020.
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Single-cell Individual Complete mtDNA Sequencing Uncovers Hidden Mitochondrial Heterogeneity in Human and Mouse Oocytes
Chongwei Bi, Lin Wang, Yong Fan, Gerardo Ramos-Mandujano, Baolei Yuan, Xuan Zhou, Jincheng Wang, Yanjiao Shao, Pu-Yao Zhang, Yanyi Huang, Yang Yu, Juan Carlos Izpisua Belmonte, Mo Li
bioRxiv 2020.12.28.424537; doi: https://doi.org/10.1101/2020.12.28.424537
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Single-cell Individual Complete mtDNA Sequencing Uncovers Hidden Mitochondrial Heterogeneity in Human and Mouse Oocytes
Chongwei Bi, Lin Wang, Yong Fan, Gerardo Ramos-Mandujano, Baolei Yuan, Xuan Zhou, Jincheng Wang, Yanjiao Shao, Pu-Yao Zhang, Yanyi Huang, Yang Yu, Juan Carlos Izpisua Belmonte, Mo Li
bioRxiv 2020.12.28.424537; doi: https://doi.org/10.1101/2020.12.28.424537

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