Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Come together: bioelectric healing-on-a-chip

View ORCID ProfileTom J. Zajdel, Gawoon Shim, View ORCID ProfileDaniel J. Cohen
doi: https://doi.org/10.1101/2020.12.29.424578
Tom J. Zajdel
aDepartment of Mechanical & Aerospace Engineering, Princeton University, Princeton, NJ, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Tom J. Zajdel
Gawoon Shim
aDepartment of Mechanical & Aerospace Engineering, Princeton University, Princeton, NJ, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniel J. Cohen
aDepartment of Mechanical & Aerospace Engineering, Princeton University, Princeton, NJ, United States of America
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Daniel J. Cohen
  • For correspondence: danielcohen@princeton.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

There is a growing interest in bioelectric wound treatment and electrotaxis, the process by which cells detect an electric field and orient their migration along its direction, has emerged as a potential cornerstone of the endogenous wound healing response. Despite recognition of the importance of electrotaxis in wound healing, no experimental system to date demonstrates that the actual closing of a wound can be accelerated solely by the electrotaxis response itself, and in vivo systems are too complex to resolve cell migration from other healing stages such as proliferation and inflammation. This uncertainty has led to a lack of standardization between stimulation methods, model systems, and electrode technology required for device development. In this paper, we present a ‘healing-on-chip’ approach that is a standardized, low-cost, model for investigating electrically accelerated wound healing. Our device provides the first convergent field geometry used in a stimulation device. We validate this device by using electrical stimulation to close a 1.5 mm gap between two large (30 mm2) primary skin keratinocyte layers to double the rate of healing over an unstimulated tissue. This proves that convergent electrotaxis is both possible and can accelerate healing, and offers a new ‘healing-on-a-chip’ platform to explore future bioelectric interfaces.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted December 30, 2020.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Come together: bioelectric healing-on-a-chip
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Come together: bioelectric healing-on-a-chip
Tom J. Zajdel, Gawoon Shim, Daniel J. Cohen
bioRxiv 2020.12.29.424578; doi: https://doi.org/10.1101/2020.12.29.424578
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Come together: bioelectric healing-on-a-chip
Tom J. Zajdel, Gawoon Shim, Daniel J. Cohen
bioRxiv 2020.12.29.424578; doi: https://doi.org/10.1101/2020.12.29.424578

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Bioengineering
Subject Areas
All Articles
  • Animal Behavior and Cognition (2430)
  • Biochemistry (4791)
  • Bioengineering (3331)
  • Bioinformatics (14675)
  • Biophysics (6637)
  • Cancer Biology (5168)
  • Cell Biology (7425)
  • Clinical Trials (138)
  • Developmental Biology (4365)
  • Ecology (6873)
  • Epidemiology (2057)
  • Evolutionary Biology (9918)
  • Genetics (7346)
  • Genomics (9527)
  • Immunology (4554)
  • Microbiology (12683)
  • Molecular Biology (4945)
  • Neuroscience (28325)
  • Paleontology (199)
  • Pathology (808)
  • Pharmacology and Toxicology (1391)
  • Physiology (2024)
  • Plant Biology (4497)
  • Scientific Communication and Education (977)
  • Synthetic Biology (1299)
  • Systems Biology (3914)
  • Zoology (726)