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Allelic variants of the NLR protein Rpi-chc1 differentially recognise members of the Phytophthora infestans PexRD12/31 effector superfamily through the leucine-rich repeat domain

Daniel Monino-Lopez, Maarten Nijenhuis, Linda Kodde, View ORCID ProfileSophien Kamoun, Hamed Salehian, Kyrylo Schentsnyi, Remco Stam, Anoma Lokossou, Ahmed Abd-El-Haliem, Richard GF Visser, View ORCID ProfileJack H Vossen
doi: https://doi.org/10.1101/2020.12.29.424692
Daniel Monino-Lopez
1Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands
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Maarten Nijenhuis
1Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands
2Agrico Research, Burchtweg 17, 8314PP, Bant, The Netherlands
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Linda Kodde
1Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands
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Sophien Kamoun
3The Sainsbury Laboratory, University of East Anglia, Norwich Research Park, Norwich, United Kingdom
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  • ORCID record for Sophien Kamoun
Hamed Salehian
1Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands
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Kyrylo Schentsnyi
1Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands
5Center for Plant Molecular Biology, Auf der Morgenstelle 32, 2076 Tübingen, Germany
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Remco Stam
1Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands
6Technical University Munich
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Anoma Lokossou
1Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands
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Ahmed Abd-El-Haliem
1Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands
8Rijk Zwaan Breeding B.V., Burgemeester Crezéelaan 40, The Netherlands
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Richard GF Visser
1Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands
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Jack H Vossen
1Plant Breeding, Wageningen University & Research, Droevendaalsesteeg 1, 6708PB, Wageningen, The Netherlands
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  • ORCID record for Jack H Vossen
  • For correspondence: jack.vossen@wur.nl
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Summary

  • Phytophthora infestans is a pathogenic oomycete that causes the infamous potato late blight disease. Resistance (R) genes from diverse Solanum species encode intracellular receptors that recognize P. infestans RXLR effector proteins and provide effective defence responses. To deploy these R genes in a durable fashion in agriculture, we need to understand the mechanism of effector recognition and the way the pathogen evades recognition.

  • We cloned sixteen allelic variants of the Rpi-chc1 gene from Solanum chacoense and other Solanum species, and identified the cognate P. infestans RXLR effectors. These tools were used to study receptor-ligand interactions and co-evolution.

  • Functional and non-functional alleles of Rpi-chc1 encode Coiled-Coil-Nucleotide Binding-Leucine-Rich-Repeat (CNL) proteins. Rpi-chc1.1 recognised multiple PexRD12 (AVRchc1.1) proteins while Rpi-chc1.2 recognised multiple PexRD31 (AVRchc1.2) proteins, both from the PexRD12/31 superfamily. Domain swaps between Rpi-chc1.1 and Rpi-chc1.2 revealed that overlapping subdomains in the LRR were responsible for the difference in effector recognition.

  • This study showed that Rpi-chc1.1 and Rpi-chc1.2, evolved to recognize distinct members of the same PexRD12/31 effector family via the LRR domain. The biased distribution of polymorphisms suggests that exchange of LRRs during host-pathogen co-evolution can lead to novel recognition specificities. These insights will help future strategies to breed for durable resistant varieties.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted December 30, 2020.
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Allelic variants of the NLR protein Rpi-chc1 differentially recognise members of the Phytophthora infestans PexRD12/31 effector superfamily through the leucine-rich repeat domain
Daniel Monino-Lopez, Maarten Nijenhuis, Linda Kodde, Sophien Kamoun, Hamed Salehian, Kyrylo Schentsnyi, Remco Stam, Anoma Lokossou, Ahmed Abd-El-Haliem, Richard GF Visser, Jack H Vossen
bioRxiv 2020.12.29.424692; doi: https://doi.org/10.1101/2020.12.29.424692
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Allelic variants of the NLR protein Rpi-chc1 differentially recognise members of the Phytophthora infestans PexRD12/31 effector superfamily through the leucine-rich repeat domain
Daniel Monino-Lopez, Maarten Nijenhuis, Linda Kodde, Sophien Kamoun, Hamed Salehian, Kyrylo Schentsnyi, Remco Stam, Anoma Lokossou, Ahmed Abd-El-Haliem, Richard GF Visser, Jack H Vossen
bioRxiv 2020.12.29.424692; doi: https://doi.org/10.1101/2020.12.29.424692

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