Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Screening a library of FDA-approved and bioactive compounds for antiviral activity against SARS-CoV-2

Scott B. Biering, Erik Van Dis, Eddie Wehri, Livia H. Yamashiro, Xammy Nguyenla, Claire Dugast-Darzacq, Thomas G.W. Graham, Julien R. Stroumza, Guillaume R. Golovkine, Allison W. Roberts, Daniel M. Fines, Jessica N. Spradlin, Carl C. Ward, Teena Bajaj, Dustin Dovala, Ursula Schulze Gahmen, Ruchika Bajaj, Douglas M. Fox, Melanie Ott, Niren Murthy, View ORCID ProfileDaniel K. Nomura, Julia Schaletzky, View ORCID ProfileSarah A. Stanley
doi: https://doi.org/10.1101/2020.12.30.424862
Scott B. Biering
1School of Public Health, Division of Infectious Diseases and Vaccinology, University of California, Berkeley, Berkeley, California, 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Erik Van Dis
2Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, California, 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eddie Wehri
3The Henry Wheeler Center for Emerging and Neglected Diseases, 344 Li Ka Shing, Berkeley, California, 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Livia H. Yamashiro
1School of Public Health, Division of Infectious Diseases and Vaccinology, University of California, Berkeley, Berkeley, California, 94720, USA
2Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, California, 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Xammy Nguyenla
1School of Public Health, Division of Infectious Diseases and Vaccinology, University of California, Berkeley, Berkeley, California, 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Claire Dugast-Darzacq
4Department of Molecular and Cell Biology, Division of Biochemistry, Biophysics and Structural Biology, University of California, Berkeley, Berkeley, California 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Thomas G.W. Graham
4Department of Molecular and Cell Biology, Division of Biochemistry, Biophysics and Structural Biology, University of California, Berkeley, Berkeley, California 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julien R. Stroumza
3The Henry Wheeler Center for Emerging and Neglected Diseases, 344 Li Ka Shing, Berkeley, California, 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Guillaume R. Golovkine
2Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, California, 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Allison W. Roberts
2Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, California, 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniel M. Fines
2Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, California, 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jessica N. Spradlin
5Departments of Chemistry, Molecular and Cell Biology, and Nutritional Sciences and Toxicology, University of California, Berkeley
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Carl C. Ward
5Departments of Chemistry, Molecular and Cell Biology, and Nutritional Sciences and Toxicology, University of California, Berkeley
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Teena Bajaj
6Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dustin Dovala
7Novartis Institutes for BioMedical Research, Emeryville, CA, 94608, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ursula Schulze Gahmen
8QBI Coronavirus Research Group Structural Biology Consortium, University of California, San Francisco, CA 94158, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ruchika Bajaj
9Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA 94158, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Douglas M. Fox
1School of Public Health, Division of Infectious Diseases and Vaccinology, University of California, Berkeley, Berkeley, California, 94720, USA
2Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, California, 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Melanie Ott
10Department of Medicine; Medical Scientist Training Program; Biomedical Sciences Graduate Program, University of California, San Francisco, San Francisco, CA 94143, USA
11J. David Gladstone Institutes, San Francisco, CA 94158, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Niren Murthy
6Department of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA
12Innovative Genomics Institute (IGI), 2151 Berkeley Way, CA 94704, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniel K. Nomura
5Departments of Chemistry, Molecular and Cell Biology, and Nutritional Sciences and Toxicology, University of California, Berkeley
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Daniel K. Nomura
Julia Schaletzky
3The Henry Wheeler Center for Emerging and Neglected Diseases, 344 Li Ka Shing, Berkeley, California, 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: sastanley@berkeley.edu
Sarah A. Stanley
1School of Public Health, Division of Infectious Diseases and Vaccinology, University of California, Berkeley, Berkeley, California, 94720, USA
2Department of Molecular and Cell Biology, Division of Immunology and Pathogenesis, University of California, Berkeley, Berkeley, California, 94720, USA
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sarah A. Stanley
  • For correspondence: sastanley@berkeley.edu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of coronavirus disease 2019 (COVID-19), has emerged as a major global health threat. The COVID-19 pandemic has resulted in over 80 million cases and 1.7 million deaths to date while the number of cases continues to rise. With limited therapeutic options, the identification of safe and effective therapeutics is urgently needed. The repurposing of known clinical compounds holds the potential for rapid identification of drugs effective against SARS-CoV-2. Here we utilized a library of FDA-approved and well-studied preclinical and clinical compounds to screen for antivirals against SARS-CoV-2 in human pulmonary epithelial cells. We identified 13 compounds that exhibit potent antiviral activity across multiple orthogonal assays. Hits include known antivirals, compounds with anti-inflammatory activity, and compounds targeting host pathways such as kinases and proteases critical for SARS-CoV-2 replication. We identified seven compounds not previously reported to have activity against SARS-CoV-2, including B02, a human RAD51 inhibitor. We further demonstrated that B02 exhibits synergy with remdesivir, the only antiviral approved by the FDA to treat COVID-19, highlighting the potential for combination therapy. Taken together, our comparative compound screening strategy highlights the potential of drug repurposing screens to identify novel starting points for development of effective antiviral mono- or combination therapies to treat COVID-19.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted December 30, 2020.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Screening a library of FDA-approved and bioactive compounds for antiviral activity against SARS-CoV-2
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Screening a library of FDA-approved and bioactive compounds for antiviral activity against SARS-CoV-2
Scott B. Biering, Erik Van Dis, Eddie Wehri, Livia H. Yamashiro, Xammy Nguyenla, Claire Dugast-Darzacq, Thomas G.W. Graham, Julien R. Stroumza, Guillaume R. Golovkine, Allison W. Roberts, Daniel M. Fines, Jessica N. Spradlin, Carl C. Ward, Teena Bajaj, Dustin Dovala, Ursula Schulze Gahmen, Ruchika Bajaj, Douglas M. Fox, Melanie Ott, Niren Murthy, Daniel K. Nomura, Julia Schaletzky, Sarah A. Stanley
bioRxiv 2020.12.30.424862; doi: https://doi.org/10.1101/2020.12.30.424862
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Screening a library of FDA-approved and bioactive compounds for antiviral activity against SARS-CoV-2
Scott B. Biering, Erik Van Dis, Eddie Wehri, Livia H. Yamashiro, Xammy Nguyenla, Claire Dugast-Darzacq, Thomas G.W. Graham, Julien R. Stroumza, Guillaume R. Golovkine, Allison W. Roberts, Daniel M. Fines, Jessica N. Spradlin, Carl C. Ward, Teena Bajaj, Dustin Dovala, Ursula Schulze Gahmen, Ruchika Bajaj, Douglas M. Fox, Melanie Ott, Niren Murthy, Daniel K. Nomura, Julia Schaletzky, Sarah A. Stanley
bioRxiv 2020.12.30.424862; doi: https://doi.org/10.1101/2020.12.30.424862

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3483)
  • Biochemistry (7336)
  • Bioengineering (5305)
  • Bioinformatics (20219)
  • Biophysics (9990)
  • Cancer Biology (7713)
  • Cell Biology (11280)
  • Clinical Trials (138)
  • Developmental Biology (6426)
  • Ecology (9927)
  • Epidemiology (2065)
  • Evolutionary Biology (13294)
  • Genetics (9353)
  • Genomics (12564)
  • Immunology (7686)
  • Microbiology (18979)
  • Molecular Biology (7426)
  • Neuroscience (40937)
  • Paleontology (300)
  • Pathology (1226)
  • Pharmacology and Toxicology (2132)
  • Physiology (3145)
  • Plant Biology (6849)
  • Scientific Communication and Education (1272)
  • Synthetic Biology (1893)
  • Systems Biology (5306)
  • Zoology (1086)