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Neutralization of N501Y mutant SARS-CoV-2 by BNT162b2 vaccine-elicited sera

Xuping Xie, Jing Zou, Camila R. Fontes-Garfias, Hongjie Xia, Kena A. Swanson, Mark Cutler, David Cooper, View ORCID ProfileVineet D. Menachery, View ORCID ProfileScott Weaver, View ORCID ProfilePhilip R. Dormitzer, Pei-Yong Shi
doi: https://doi.org/10.1101/2021.01.07.425740
Xuping Xie
1Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston TX, U.S.A.
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Jing Zou
1Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston TX, U.S.A.
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Camila R. Fontes-Garfias
1Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston TX, U.S.A.
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Hongjie Xia
1Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston TX, U.S.A.
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Kena A. Swanson
2Pfizer, 401 N Middletown Rd., Pearl River, NY 10960, U.S.A.
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Mark Cutler
2Pfizer, 401 N Middletown Rd., Pearl River, NY 10960, U.S.A.
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David Cooper
2Pfizer, 401 N Middletown Rd., Pearl River, NY 10960, U.S.A.
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Vineet D. Menachery
3Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston TX, U.S.A.
4Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, TX, USA
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  • ORCID record for Vineet D. Menachery
Scott Weaver
3Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston TX, U.S.A.
4Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, TX, USA
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Philip R. Dormitzer
2Pfizer, 401 N Middletown Rd., Pearl River, NY 10960, U.S.A.
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  • ORCID record for Philip R. Dormitzer
  • For correspondence: Philip.Dormitzer@pfizer.com peshi@UTMB.edu
Pei-Yong Shi
1Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston TX, U.S.A.
4Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, TX, USA
5Institute for Translational Sciences, University of Texas Medical Branch, Galveston, TX, U.S.A.
6Sealy Institute for Vaccine Sciences, University of Texas Medical Branch, Galveston, TX, U.S.A.
7Sealy Center for Structural Biology & Molecular Biophysics, University of Texas Medical Branch, Galveston, TX, U.S.A.
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  • For correspondence: Philip.Dormitzer@pfizer.com peshi@UTMB.edu
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Abstract

Rapidly spreading variants of SARS-CoV-2 that have arisen in the United Kingdom and South Africa share the spike N501Y substitution, which is of particular concern because it is located in the viral receptor binding site for cell entry and increases binding to the receptor (angiotensin converting enzyme 2). We generated isogenic N501 and Y501 SARS-CoV-2. Sera of 20 participants in a previously reported trial of the mRNA-based COVID-19 vaccine BNT162b2 had equivalent neutralizing titers to the N501 and Y501 viruses.

Competing Interest Statement

X.X., V.D.M., and P.-Y.S. have filed a patent on the reverse genetic system. K.A.S., M.C., D.C., and P.R.D. are employees of Pfizer and may hold stock options. X.P., J.Z., C.R.F.G., H.X., and P.-Y.S. received compensation from Pfizer to perform the neutralization assay.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 07, 2021.
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Neutralization of N501Y mutant SARS-CoV-2 by BNT162b2 vaccine-elicited sera
Xuping Xie, Jing Zou, Camila R. Fontes-Garfias, Hongjie Xia, Kena A. Swanson, Mark Cutler, David Cooper, Vineet D. Menachery, Scott Weaver, Philip R. Dormitzer, Pei-Yong Shi
bioRxiv 2021.01.07.425740; doi: https://doi.org/10.1101/2021.01.07.425740
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Neutralization of N501Y mutant SARS-CoV-2 by BNT162b2 vaccine-elicited sera
Xuping Xie, Jing Zou, Camila R. Fontes-Garfias, Hongjie Xia, Kena A. Swanson, Mark Cutler, David Cooper, Vineet D. Menachery, Scott Weaver, Philip R. Dormitzer, Pei-Yong Shi
bioRxiv 2021.01.07.425740; doi: https://doi.org/10.1101/2021.01.07.425740

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