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In Vivo Sublayer Analysis Of Human Retinal Inner Plexiform Layer Obtained By Visible-Light Optical Coherence Tomography

Zeinab Ghassabi, Roman V. Kuranov, Mengfei Wu, Behnam Tayebi, Yuanbo Wang, Ian Rubinoff, View ORCID ProfileXiaorong Liu, Gadi Wollstein, View ORCID ProfileJoel S. Schuman, View ORCID ProfileHao F. Zhang, View ORCID ProfileHiroshi Ishikawa
doi: https://doi.org/10.1101/2021.01.08.425925
Zeinab Ghassabi
1Department of Ophthalmology, NYU Langone Health, New York, NY, United States
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Roman V. Kuranov
2Department of Biomedical Engineering, Northwestern University, Evanston, IL, United States
3Opticent Inc., Evanston, IL, United States
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Mengfei Wu
1Department of Ophthalmology, NYU Langone Health, New York, NY, United States
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Behnam Tayebi
1Department of Ophthalmology, NYU Langone Health, New York, NY, United States
4Neuroscience Institute, NYU Langone Health, NY, United States
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Yuanbo Wang
3Opticent Inc., Evanston, IL, United States
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Ian Rubinoff
2Department of Biomedical Engineering, Northwestern University, Evanston, IL, United States
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Xiaorong Liu
5Department of Biology, University of Virginia, Charlottesville, VA, United States
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Gadi Wollstein
1Department of Ophthalmology, NYU Langone Health, New York, NY, United States
6Department of Biomedical Engineering, New York University Tandon School of Engineering, New York, NY, United States
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Joel S. Schuman
1Department of Ophthalmology, NYU Langone Health, New York, NY, United States
6Department of Biomedical Engineering, New York University Tandon School of Engineering, New York, NY, United States
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Hao F. Zhang
2Department of Biomedical Engineering, Northwestern University, Evanston, IL, United States
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Hiroshi Ishikawa
1Department of Ophthalmology, NYU Langone Health, New York, NY, United States
6Department of Biomedical Engineering, New York University Tandon School of Engineering, New York, NY, United States
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  • ORCID record for Hiroshi Ishikawa
  • For correspondence: hiroshi.ishikawa@nyulangone.org
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Abstract

Purpose Growing evidence suggests, in glaucoma, the dendritic degeneration of subpopulation of the retinal ganglion cells (RGCs) may precede RGCs soma death. Since different RGCs synapse in different IPL sublayers, visualization of the lamellar structure of the IPL could enable both clinical and fundamental advances in glaucoma understanding and management. In this pilot study, we investigated whether visible-light optical coherence tomography (vis-OCT) could detect the difference in the inner plexiform layer (IPL) sublayers thicknesses between small cohorts of healthy and glaucomatous subjects.

Method We investigated vis-OCT retinal images from nine healthy and five glaucomatous subjects. Four of the healthy subjects were scanned three times each in two separate visits, and five healthy and five glaucoma subjects were scanned three times during a single visit. Raster speckle-reduction scans (3 by 3 by 1.2 mm^3: horizontal; vertical; axial directions with 8192×8×1024 samplings, respectively) of the superior macular were acquired. IPL sublayers were then manually segmented using averaged A-line profiles.

Results The mean ages of glaucoma and healthy subjects are 59.6 +/- 13.4 and 45.4 +/- 14.4 years (p =0.02, Wilcoxon rank-sum test), respectively. The visual field mean deviation (MD) are −26.4 to −7.7 dB in glaucoma patient and −1.6 to 1.1 dB in healthy subjects (p =0.002). The mean circumpapillary retinal nerve fiber layer (RNFL) thicknesses are 59.6 +/- 9.1 μm in glaucoma and 99.2 +/- 16.2 μm in healthy subjects (p=0.004). Median coefficients of variation (CVs) of intra-session repeatability for the entire IPL and three sublayers are 3.1%, 5.6%, 6.9%, and 5.6% in healthy subjects and 1.8%, 6.0%, 7.7%, and 6.2% in glaucoma patients, respectively. The mean entire IPL thicknesses are 36.2 +/- 1.5 μm in glaucomatous and 40.1 +/- 1.7 micrometer in healthy eyes (p=0.003, Mixed-effects model). We found that the middle sublayer thickness was responsible for the majority of the difference (14.2 +/- 1.8 μm in glaucomatous and 17.5 +/- 1.4 in healthy eyes, p<0.01).

Conclusions IPL sublayer analysis revealed that the middle sublayer could be responsible for the majority of IPL thinning in glaucoma. Vis-OCT quantified IPL sublayers with good repeatability in both glaucoma and healthy subjects. Visualization of the IPL sublayers may enable the investigation of lamella-specific changes in the IPL in glaucoma and may help elucidate the response of different types of RGCs to the disease.

Competing Interest Statement

Roman V. Kuranov, Yuanbo Wang, Hao F. Zhang, and Joel S. Schuman have financial interests in Opticent Inc.

Footnotes

  • The financial interests of Roman V. Kuranov, Yuanbo Wang, Hao F. Zhang, and Joel S Schuman with Opticent Inc. were indicated.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted January 10, 2021.
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In Vivo Sublayer Analysis Of Human Retinal Inner Plexiform Layer Obtained By Visible-Light Optical Coherence Tomography
Zeinab Ghassabi, Roman V. Kuranov, Mengfei Wu, Behnam Tayebi, Yuanbo Wang, Ian Rubinoff, Xiaorong Liu, Gadi Wollstein, Joel S. Schuman, Hao F. Zhang, Hiroshi Ishikawa
bioRxiv 2021.01.08.425925; doi: https://doi.org/10.1101/2021.01.08.425925
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In Vivo Sublayer Analysis Of Human Retinal Inner Plexiform Layer Obtained By Visible-Light Optical Coherence Tomography
Zeinab Ghassabi, Roman V. Kuranov, Mengfei Wu, Behnam Tayebi, Yuanbo Wang, Ian Rubinoff, Xiaorong Liu, Gadi Wollstein, Joel S. Schuman, Hao F. Zhang, Hiroshi Ishikawa
bioRxiv 2021.01.08.425925; doi: https://doi.org/10.1101/2021.01.08.425925

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