Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

MultipleTesting.com: a tool for life science researchers for multiple hypothesis testing correction

View ORCID ProfileOtília Menyhárt, Boglárka Weltz, Balázs Győrffy
doi: https://doi.org/10.1101/2021.01.11.426197
Otília Menyhárt
1Semmelweis University, Department of Bioinformatics, H-1094, Budapest, Hungary
2Research Centre for Natural Sciences, Cancer Biomarker Research Group, Institute of Enzymology, H-1117, Budapest, Hungary
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Otília Menyhárt
Boglárka Weltz
2Research Centre for Natural Sciences, Cancer Biomarker Research Group, Institute of Enzymology, H-1117, Budapest, Hungary
3A5 Genetics Ltd, H-9464, Und, Hungary
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Balázs Győrffy
1Semmelweis University, Department of Bioinformatics, H-1094, Budapest, Hungary
2Research Centre for Natural Sciences, Cancer Biomarker Research Group, Institute of Enzymology, H-1117, Budapest, Hungary
4nd, H-1094, Budapest, Hungary
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: gyorffy.balazs@med.semmelweis-univ.hu
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

ABSTRACT

Scientists from nearly all disciplines face the problem of simultaneously evaluating many hypotheses. Conducting multiple comparisons increases the likelihood that a non-negligible proportion of associations will be false positives, clouding real discoveries.

Drawing valid conclusions require taking into account the number of performed statistical tests and adjusting the statistical confidence measures. Several strategies exist to overcome the problem of multiple hypothesis testing. We aim to summarize critical statistical concepts and widely used correction approaches while also draw attention to frequently misinterpreted notions of statistical inference.

We provide a step-by-step description of each multiple-testing correction method with clear examples and present an easy-to-follow guide for selecting the most suitable correction technique.

To facilitate multiple-testing corrections, we developed a fully automated solution not requiring programming skills or the use of a command line. Our registration free online tool is available at www.multipletesting.com and compiles the five most frequently used adjustment tools, including the Bonferroni, the Holm (step-down), the Hochberg (step-up) corrections, allows to calculate False Discovery Rates (FDR) and q-values.

The current summary provides a much needed practical synthesis of basic statistical concepts regarding multiple hypothesis testing in a comprehensible language with well-illustrated examples. The web tool will fill the gap for life science researchers by providing a user-friendly substitute for command-line alternatives.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY 4.0 International license.
Back to top
PreviousNext
Posted January 11, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
MultipleTesting.com: a tool for life science researchers for multiple hypothesis testing correction
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
MultipleTesting.com: a tool for life science researchers for multiple hypothesis testing correction
Otília Menyhárt, Boglárka Weltz, Balázs Győrffy
bioRxiv 2021.01.11.426197; doi: https://doi.org/10.1101/2021.01.11.426197
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
MultipleTesting.com: a tool for life science researchers for multiple hypothesis testing correction
Otília Menyhárt, Boglárka Weltz, Balázs Győrffy
bioRxiv 2021.01.11.426197; doi: https://doi.org/10.1101/2021.01.11.426197

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Bioinformatics
Subject Areas
All Articles
  • Animal Behavior and Cognition (4095)
  • Biochemistry (8784)
  • Bioengineering (6490)
  • Bioinformatics (23380)
  • Biophysics (11765)
  • Cancer Biology (9166)
  • Cell Biology (13281)
  • Clinical Trials (138)
  • Developmental Biology (7421)
  • Ecology (11381)
  • Epidemiology (2066)
  • Evolutionary Biology (15111)
  • Genetics (10408)
  • Genomics (14017)
  • Immunology (9136)
  • Microbiology (22088)
  • Molecular Biology (8792)
  • Neuroscience (47421)
  • Paleontology (350)
  • Pathology (1422)
  • Pharmacology and Toxicology (2483)
  • Physiology (3710)
  • Plant Biology (8060)
  • Scientific Communication and Education (1433)
  • Synthetic Biology (2213)
  • Systems Biology (6020)
  • Zoology (1251)