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Distinct Patterns of Emergence of SARS-CoV-2 Spike Variants including N501Y in Clinical Samples in Columbus Ohio

Huolin Tu, Matthew R Avenarius, Laura Kubatko, Matthew Hunt, Xiaokang Pan, Peng Ru, Jason Garee, Keelie Thomas, Peter Mohler, Preeti Pancholi, Dan Jones
doi: https://doi.org/10.1101/2021.01.12.426407
Huolin Tu
1James Molecular Laboratory at Polaris, The Ohio State University James Cancer Center, Columbus, OH, USA
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Matthew R Avenarius
1James Molecular Laboratory at Polaris, The Ohio State University James Cancer Center, Columbus, OH, USA
2Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH, USA
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Laura Kubatko
3Department of Evolution, Ecology and Organismal Biology, The Ohio State University James Cancer Center, Columbus, OH, USA
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Matthew Hunt
1James Molecular Laboratory at Polaris, The Ohio State University James Cancer Center, Columbus, OH, USA
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Xiaokang Pan
1James Molecular Laboratory at Polaris, The Ohio State University James Cancer Center, Columbus, OH, USA
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Peng Ru
4The Ohio State University Comprehensive Cancer Center, The Ohio State University James Cancer Center, Columbus, OH, USA
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Jason Garee
1James Molecular Laboratory at Polaris, The Ohio State University James Cancer Center, Columbus, OH, USA
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Keelie Thomas
2Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH, USA
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Peter Mohler
5Departments of Physiology and Internal Medicine and Davis Heart and Lung Research Institute, The College of Medicine and Ohio State University Wexner Medical Center
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Preeti Pancholi
2Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH, USA
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Dan Jones
1James Molecular Laboratory at Polaris, The Ohio State University James Cancer Center, Columbus, OH, USA
2Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH, USA
4The Ohio State University Comprehensive Cancer Center, The Ohio State University James Cancer Center, Columbus, OH, USA
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  • For correspondence: daniel.jones@osumc.edu
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Abstract

Following the worldwide emergence of the p.Asp614Gly shift in the Spike (S) gene of SARS-CoV-2, there have been few recurring pathogenic shifts occurring during 2020, as assessed by genomic sequencing. This situation has evolved in the last several months with the emergence of several distinct variants (first identified in the United Kingdom and South Africa, respectively) that illustrate multiple changes in the S gene, particularly p.Asn501Tyr (N501Y), that likely have clinical impact. We report here the emergence in Columbus, Ohio in December 2020 of two novel SARS-CoV-2 clade 20C/G variants. One isolate, that has become the predominant virus found in nasopharyngeal swabs in the December 2020-January 2021 period, harbors S p.Gln677His, membrane glycoprotein (M) p.Ala85Ser (Q677H) and nucleocapsid (N) p.Asp377Tyr (D377Y) mutations. The other isolate contains S N501Y and ORF8 Arg52Ile (R52I), which are two markers of the UK-B.1.1.7 (clade 20I/501Y.V1) strain, but lacks all other mutations from that virus. It is also from a different clade and shares multiple mutations with the clade 20C/G viruses circulating in Ohio prior to December 2020. These two SARS-CoV-2 viruses emerging now in the United States add to the diversity of S gene shifts occurring worldwide and support multiple independent acquisition of S N501Y (in likely contrast to the unitary S D614G shift) occurring first during this period of the pandemic.

Competing Interest Statement

The authors have declared no competing interest.

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Posted January 15, 2021.
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Distinct Patterns of Emergence of SARS-CoV-2 Spike Variants including N501Y in Clinical Samples in Columbus Ohio
Huolin Tu, Matthew R Avenarius, Laura Kubatko, Matthew Hunt, Xiaokang Pan, Peng Ru, Jason Garee, Keelie Thomas, Peter Mohler, Preeti Pancholi, Dan Jones
bioRxiv 2021.01.12.426407; doi: https://doi.org/10.1101/2021.01.12.426407
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Distinct Patterns of Emergence of SARS-CoV-2 Spike Variants including N501Y in Clinical Samples in Columbus Ohio
Huolin Tu, Matthew R Avenarius, Laura Kubatko, Matthew Hunt, Xiaokang Pan, Peng Ru, Jason Garee, Keelie Thomas, Peter Mohler, Preeti Pancholi, Dan Jones
bioRxiv 2021.01.12.426407; doi: https://doi.org/10.1101/2021.01.12.426407

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