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Anti-CoVid19 plasmid DNA vaccine induces a potent immune response in rodents by Pyro-drive Jet Injector intradermal inoculation

Tomoyuki Nishikawa, Chin Yang Chang, Jiayu A Tai, Hiroki Hayashi, Jiao Sun, Shiho Torii, Chikako Ono, View ORCID ProfileYoshiharu Matsuura, Ryoko Ide, Junichi Mineno, Miwa Sasai, Masahiro Yamamoto, Hironori Nakagami, Kunihiko Yamashita
doi: https://doi.org/10.1101/2021.01.13.426436
Tomoyuki Nishikawa
1Department of Device Application for Molecular Therapeutics, Graduate School of Medicine, Osaka University, Japan
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Chin Yang Chang
1Department of Device Application for Molecular Therapeutics, Graduate School of Medicine, Osaka University, Japan
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Jiayu A Tai
1Department of Device Application for Molecular Therapeutics, Graduate School of Medicine, Osaka University, Japan
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Hiroki Hayashi
2Department of Health Development and Medicine, Graduate School of Medicine, Osaka University, Japan
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Jiao Sun
2Department of Health Development and Medicine, Graduate School of Medicine, Osaka University, Japan
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Shiho Torii
3Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Japan
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Chikako Ono
3Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Japan
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Yoshiharu Matsuura
3Department of Molecular Virology, Research Institute for Microbial Diseases, Osaka University, Japan
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  • ORCID record for Yoshiharu Matsuura
Ryoko Ide
4FunPep Co., Ltd., Japan
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Junichi Mineno
5Takara Bio Inc., Japan
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Miwa Sasai
6Department of Immunoparasitology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
7Laboratory of Immunoparasitology, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
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Masahiro Yamamoto
6Department of Immunoparasitology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan
7Laboratory of Immunoparasitology, WPI Immunology Frontier Research Center, Osaka University, Osaka, Japan
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Hironori Nakagami
2Department of Health Development and Medicine, Graduate School of Medicine, Osaka University, Japan
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Kunihiko Yamashita
1Department of Device Application for Molecular Therapeutics, Graduate School of Medicine, Osaka University, Japan
8Medical Device Research, Industry Business Unit, Safety Strategic Business unit, Daicel Corporation, Japan
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  • For correspondence: ku_yamashita@jp.daicel.com
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Abstract

There is an urgent need to limit and stop the worldwide coronavirus disease 2019 (COVID-19) pandemic via quick development of efficient and safe vaccination methods. Plasmid DNA vaccines are one of the most remarkable vaccines that can be developed in a short term. pVAX1-SARS-CoV2-co, which is a plasmid DNA vaccine, was designed to express severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) spike protein. The produced antibodies lead to Immunoreactions against S protein, anti-receptor-binding-domain, and neutralizing action of pVAX1-SARS-CoV2-co, as confirmed in a previous study. To promote the efficacy of the pVAX1-SARS-CoV2-co vaccine, a pyro-drive jet injector (PJI) was employed. PJI is an injection device that can adjust the injection pressure depending on various target tissues. Intradermally-adjusted PJI demonstrated that pVAX1-SARS-CoV2-co vaccine injection caused a strong production of anti-S protein antibodies, triggered immunoreactions and neutralizing actions against SARS-CoV-2. Moreover, a high dose of pVAX1-SARS-CoV2-co intradermal injection via PJI did not cause any serious disorders in the rat model. Finally, virus infection challenge in mice, confirmed that intradermally immunized (via PJI) mice were potently protected from COVID-19 infection. Thus, pVAX1-SARS-CoV2-co intradermal injection via PJI is a safe and promising vaccination method to overcome the COVID-19 pandemic.

Competing Interest Statement

The Department of Device Application for Molecular Therapeutics is a joint research department supported by Daicel Co. The Department of Health Development and Medicine, Graduate School of Medicine is an endowed department supported by Anges, Daicel and FunPep. R.I, J.M., and K.Y. are employees of FunPep Co., Takara Bio Inc., and Daicel Co. respectively. All other authors declare no competing interest.

Footnotes

  • Competing Interest Statement: The Department of Device Application for Molecular Therapeutics is a joint research department supported by Daicel Co. The Department of Health Development and Medicine, Graduate School of Medicine is an endowed department supported by Anges, Daicel and FunPep. R.I, J.M., and K.Y. are employees of FunPep Co., Takara Bio Inc., and Daicel Co. respectively. All other authors declare no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted January 14, 2021.
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Anti-CoVid19 plasmid DNA vaccine induces a potent immune response in rodents by Pyro-drive Jet Injector intradermal inoculation
Tomoyuki Nishikawa, Chin Yang Chang, Jiayu A Tai, Hiroki Hayashi, Jiao Sun, Shiho Torii, Chikako Ono, Yoshiharu Matsuura, Ryoko Ide, Junichi Mineno, Miwa Sasai, Masahiro Yamamoto, Hironori Nakagami, Kunihiko Yamashita
bioRxiv 2021.01.13.426436; doi: https://doi.org/10.1101/2021.01.13.426436
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Anti-CoVid19 plasmid DNA vaccine induces a potent immune response in rodents by Pyro-drive Jet Injector intradermal inoculation
Tomoyuki Nishikawa, Chin Yang Chang, Jiayu A Tai, Hiroki Hayashi, Jiao Sun, Shiho Torii, Chikako Ono, Yoshiharu Matsuura, Ryoko Ide, Junichi Mineno, Miwa Sasai, Masahiro Yamamoto, Hironori Nakagami, Kunihiko Yamashita
bioRxiv 2021.01.13.426436; doi: https://doi.org/10.1101/2021.01.13.426436

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