Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Protein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages

View ORCID ProfileMohammad Zeeshan, Rajan Pandey, View ORCID ProfileAmit Kumar Subudhi, View ORCID ProfileDavid J. P. Ferguson, Gursimran Kaur, Ravish Rashpa, Raushan Nugmanova, View ORCID ProfileDeclan Brady, View ORCID ProfileAndrew R. Bottrill, View ORCID ProfileSue Vaughan, View ORCID ProfileMathieu Brochet, View ORCID ProfileMathieu Bollen, View ORCID ProfileArnab Pain, View ORCID ProfileAnthony A. Holder, View ORCID ProfileDavid S. Guttery, View ORCID ProfileRita Tewari
doi: https://doi.org/10.1101/2021.01.15.426883
Mohammad Zeeshan
1School of Life Sciences, University of Nottingham, Nottingham, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mohammad Zeeshan
Rajan Pandey
1School of Life Sciences, University of Nottingham, Nottingham, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Amit Kumar Subudhi
2Pathogen Genomics Group, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Amit Kumar Subudhi
David J. P. Ferguson
3Nuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK
4Department of Biological and Medical Sciences, Faculty of Health and Life Science, Oxford Brookes University, Gipsy Lane, Oxford, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for David J. P. Ferguson
Gursimran Kaur
1School of Life Sciences, University of Nottingham, Nottingham, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Ravish Rashpa
5Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Raushan Nugmanova
2Pathogen Genomics Group, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Declan Brady
1School of Life Sciences, University of Nottingham, Nottingham, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Declan Brady
Andrew R. Bottrill
6School of Life Sciences, Gibbet Hill Campus, University of Warwick, Coventry CV4 7AL, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Andrew R. Bottrill
Sue Vaughan
4Department of Biological and Medical Sciences, Faculty of Health and Life Science, Oxford Brookes University, Gipsy Lane, Oxford, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sue Vaughan
Mathieu Brochet
5Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mathieu Brochet
Mathieu Bollen
7Laboratory of Biosignaling and Therapeutics, KU Leuven Department of Cellular and Molecular Medicine, University of Leuven, B-3000 Leuven, Belgium
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mathieu Bollen
Arnab Pain
2Pathogen Genomics Group, BESE Division, King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia
8Research Center for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE); Hokkaido University, Sapporo, Japan
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Arnab Pain
Anthony A. Holder
9Malaria Parasitology Laboratory, The Francis Crick Institute, London, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Anthony A. Holder
David S. Guttery
1School of Life Sciences, University of Nottingham, Nottingham, UK
10Leicester Cancer Research Centre, University of Leicester, Leicester, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for David S. Guttery
Rita Tewari
1School of Life Sciences, University of Nottingham, Nottingham, UK
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Rita Tewari
  • For correspondence: rita.tewari@nottingham.ac.uk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

PP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the parasite life cycle include sexual development within the mosquito vector, with male gamete formation characterized by an atypical rapid mitosis, consisting of three rounds of DNA synthesis, successive spindle formation with clustered kinetochores, and a meiotic stage during zygote to ookinete development following fertilization. It is unclear how PP1 is involved in these unusual processes. Using real-time live-cell and ultrastructural imaging, conditional gene knockdown, RNA-seq and proteomic approaches, we show that Plasmodium PP1 is involved in both chromosome segregation during mitotic exit, and establishment of cell polarity during zygote development in the mosquito midgut, suggesting that small molecule inhibitors of PP1 should be explored for blocking parasite transmission.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted January 17, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Protein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Protein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages
Mohammad Zeeshan, Rajan Pandey, Amit Kumar Subudhi, David J. P. Ferguson, Gursimran Kaur, Ravish Rashpa, Raushan Nugmanova, Declan Brady, Andrew R. Bottrill, Sue Vaughan, Mathieu Brochet, Mathieu Bollen, Arnab Pain, Anthony A. Holder, David S. Guttery, Rita Tewari
bioRxiv 2021.01.15.426883; doi: https://doi.org/10.1101/2021.01.15.426883
Digg logo Reddit logo Twitter logo CiteULike logo Facebook logo Google logo Mendeley logo
Citation Tools
Protein Phosphatase 1 regulates atypical chromosome segregation and cell polarity during mitotic and meiotic division in Plasmodium sexual stages
Mohammad Zeeshan, Rajan Pandey, Amit Kumar Subudhi, David J. P. Ferguson, Gursimran Kaur, Ravish Rashpa, Raushan Nugmanova, Declan Brady, Andrew R. Bottrill, Sue Vaughan, Mathieu Brochet, Mathieu Bollen, Arnab Pain, Anthony A. Holder, David S. Guttery, Rita Tewari
bioRxiv 2021.01.15.426883; doi: https://doi.org/10.1101/2021.01.15.426883

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (2646)
  • Biochemistry (5259)
  • Bioengineering (3671)
  • Bioinformatics (15788)
  • Biophysics (7247)
  • Cancer Biology (5624)
  • Cell Biology (8088)
  • Clinical Trials (138)
  • Developmental Biology (4763)
  • Ecology (7510)
  • Epidemiology (2059)
  • Evolutionary Biology (10571)
  • Genetics (7727)
  • Genomics (10125)
  • Immunology (5187)
  • Microbiology (13896)
  • Molecular Biology (5383)
  • Neuroscience (30754)
  • Paleontology (215)
  • Pathology (876)
  • Pharmacology and Toxicology (1524)
  • Physiology (2253)
  • Plant Biology (5017)
  • Scientific Communication and Education (1040)
  • Synthetic Biology (1384)
  • Systems Biology (4145)
  • Zoology (811)