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The intrinsic chaperone network of Arabidopsis stem cells confers protection against proteotoxic stress

View ORCID ProfileErnesto Llamas, View ORCID ProfileSalvador Torres-Montilla, Hyun Ju Lee, María Victoria Barja, Elena Schlimgen, Nick Dunken, Prerana Wagle, Wolfgang Werr, View ORCID ProfileAlga Zuccaro, View ORCID ProfileManuel Rodríguez-Concepción, View ORCID ProfileDavid Vilchez
doi: https://doi.org/10.1101/2021.01.19.427268
Ernesto Llamas
1Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Cologne, Germany
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  • ORCID record for Ernesto Llamas
Salvador Torres-Montilla
2Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB Bellaterra, 08193 Barcelona, Spain
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Hyun Ju Lee
1Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Cologne, Germany
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María Victoria Barja
2Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB Bellaterra, 08193 Barcelona, Spain
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Elena Schlimgen
1Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Cologne, Germany
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Nick Dunken
3Cluster of Excellence on Plant Sciences (CEPLAS), Institute for Plant Sciences, University of Cologne, D-50674 Cologne, Germany
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Prerana Wagle
1Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Cologne, Germany
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Wolfgang Werr
4Developmental Biology, Biocenter, University of Cologne, D-50674 Cologne, Germany
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Alga Zuccaro
3Cluster of Excellence on Plant Sciences (CEPLAS), Institute for Plant Sciences, University of Cologne, D-50674 Cologne, Germany
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Manuel Rodríguez-Concepción
2Centre for Research in Agricultural Genomics (CRAG) CSIC-IRTA-UAB-UB, Campus UAB Bellaterra, 08193 Barcelona, Spain
5Institute for Plant Molecular and Cell Biology (IBMCP) CSIC-UPV, 46022 Valencia, Spain
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David Vilchez
1Cologne Excellence Cluster for Cellular Stress Responses in Aging-Associated Diseases (CECAD), University of Cologne, 50931 Cologne, Germany
6Center for Molecular Medicine Cologne (CMMC), University of Cologne, 50931 Cologne, Germany
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  • For correspondence: dvilchez@uni-koeln.de
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Abstract

The biological purpose of plant stem cells is to maintain themselves while providing new pools of differentiated cells that form organs and rejuvenate or replace damaged tissues1-3. Protein homeostasis, or proteostasis, is required for cell function and viability4-7. However, the link between proteostasis and plant stem cell identity remains unknown. In contrast to their differentiated counterparts, we find that root stem cells can prevent the accumulation of aggregated proteins even under proteotoxic stress conditions such as heat stress or proteasome inhibition. Notably, root stem cells exhibit enhanced expression of distinct chaperones that maintain proteome integrity. Particularly, intrinsic high levels of the TRiC/CCT chaperonin determine stem cell maintenance and their remarkable ability to suppress protein aggregation. Overexpression of CCT8, a key activator of TRiC/CCT assembly8, is sufficient to ameliorate protein aggregation in differentiated cells and confer resistance to proteotoxic stress in plants. Taken together, our results indicate that enhanced proteostasis mechanisms in stem cells could be an important requirement for plants to persist under extreme environmental conditions and reach extreme long ages. Thus, proteostasis of stem cells could provide insights to design and breed plants tolerant to environmental challenges caused by the climate change.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 20, 2021.
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The intrinsic chaperone network of Arabidopsis stem cells confers protection against proteotoxic stress
Ernesto Llamas, Salvador Torres-Montilla, Hyun Ju Lee, María Victoria Barja, Elena Schlimgen, Nick Dunken, Prerana Wagle, Wolfgang Werr, Alga Zuccaro, Manuel Rodríguez-Concepción, David Vilchez
bioRxiv 2021.01.19.427268; doi: https://doi.org/10.1101/2021.01.19.427268
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The intrinsic chaperone network of Arabidopsis stem cells confers protection against proteotoxic stress
Ernesto Llamas, Salvador Torres-Montilla, Hyun Ju Lee, María Victoria Barja, Elena Schlimgen, Nick Dunken, Prerana Wagle, Wolfgang Werr, Alga Zuccaro, Manuel Rodríguez-Concepción, David Vilchez
bioRxiv 2021.01.19.427268; doi: https://doi.org/10.1101/2021.01.19.427268

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