Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms

Francois Iv, Carla Silva Martins, Gerard Castro-Linares, View ORCID ProfileCyntia Taveneau, View ORCID ProfilePascale Barbier, View ORCID ProfilePascal Verdier-Pinard, View ORCID ProfileLuc Camoin, View ORCID ProfileStéphane Audebert, View ORCID ProfileFeng-Ching Tsai, Laurie Ramond, Alex Llewellyn, Mayssa Belhabib, Koyomi Nakazawa, Aurélie Di Cicco, Renaud Vincentelli, Jerome Wenger, View ORCID ProfileStéphanie Cabantous, View ORCID ProfileGijsje H. Koenderink, View ORCID ProfileAurélie Bertin, View ORCID ProfileManos Mavrakis
doi: https://doi.org/10.1101/2021.01.21.427698
Francois Iv
1Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 13013 Marseille, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Carla Silva Martins
1Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 13013 Marseille, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Gerard Castro-Linares
2Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, 2629 HZ Delft, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cyntia Taveneau
3Institut Curie, Université PSL, Sorbonne Université, CNRS UMR 168, Laboratoire Physico Chimie Curie,75005 Paris, France
4ARC Centre of Excellence in Advanced Molecular Imaging, Monash University, Australia; Biomedicine Discovery Institute, Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Cyntia Taveneau
Pascale Barbier
5Aix-Marseille Univ, CNRS, UMR 7051, Institut de Neurophysiopathologie (INP), Marseille, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Pascale Barbier
Pascal Verdier-Pinard
6Centre de Recherche en Cancérologie de Marseille (CRCM), INSERM, Institut Paoli-Calmettes, Aix Marseille Univ, CNRS, 13009 Marseille, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Pascal Verdier-Pinard
Luc Camoin
7Aix-Marseille Univ, INSERM, CNRS, Institut Paoli-Calmettes, CRCM, Marseille Protéomique, Marseille, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Luc Camoin
Stéphane Audebert
7Aix-Marseille Univ, INSERM, CNRS, Institut Paoli-Calmettes, CRCM, Marseille Protéomique, Marseille, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Stéphane Audebert
Feng-Ching Tsai
8Department of Living Matter, AMOLF, 1098 XG Amsterdam, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Feng-Ching Tsai
Laurie Ramond
1Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 13013 Marseille, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alex Llewellyn
1Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 13013 Marseille, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mayssa Belhabib
1Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 13013 Marseille, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Koyomi Nakazawa
3Institut Curie, Université PSL, Sorbonne Université, CNRS UMR 168, Laboratoire Physico Chimie Curie,75005 Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Aurélie Di Cicco
3Institut Curie, Université PSL, Sorbonne Université, CNRS UMR 168, Laboratoire Physico Chimie Curie,75005 Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Renaud Vincentelli
9Architecture et Fonction des Macromolécules Biologiques (AFMB), CNRS UMR7257, Aix Marseille Univ, 13009 Marseille, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jerome Wenger
1Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 13013 Marseille, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Stéphanie Cabantous
10Centre de Recherche en Cancérologie de Toulouse (CRCT), Inserm, Université Paul Sabatier-Toulouse III, CNRS, 31037 Toulouse, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Stéphanie Cabantous
Gijsje H. Koenderink
2Department of Bionanoscience, Kavli Institute of Nanoscience Delft, Delft University of Technology, 2629 HZ Delft, The Netherlands
8Department of Living Matter, AMOLF, 1098 XG Amsterdam, The Netherlands
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Gijsje H. Koenderink
  • For correspondence: manos.mavrakis@univ-amu.fr aurelie.bertin@curie.fr G.H.Koenderink@tudelft.nl
Aurélie Bertin
3Institut Curie, Université PSL, Sorbonne Université, CNRS UMR 168, Laboratoire Physico Chimie Curie,75005 Paris, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Aurélie Bertin
  • For correspondence: manos.mavrakis@univ-amu.fr aurelie.bertin@curie.fr G.H.Koenderink@tudelft.nl
Manos Mavrakis
1Institut Fresnel, CNRS UMR7249, Aix Marseille Univ, Centrale Marseille, 13013 Marseille, France
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Manos Mavrakis
  • For correspondence: manos.mavrakis@univ-amu.fr aurelie.bertin@curie.fr G.H.Koenderink@tudelft.nl
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

Abstract

Septin GTP-binding proteins contribute essential biological functions that range from the establishment of cell polarity to animal tissue morphogenesis. Human septins in cells form hetero-octameric septin complexes containing the ubiquitously expressed SEPT9. Despite the established role of SEPT9 in mammalian development and human pathophysiology, biochemical and biophysical studies have relied on monomeric SEPT9 thus not recapitulating its native assembly into hetero-octameric complexes. We established a protocol that enabled the first-time isolation of recombinant human septin octamers containing distinct SEPT9 isoforms. A combination of biochemical and biophysical assays confirmed the octameric nature of the isolated complexes in solution. Reconstitution studies showed that octamers with either a long or a short SEPT9 isoform form filament assemblies, and can directly bind and cross-link actin filaments, raising the possibility that septin-decorated actin structures in cells reflect direct actin-septin interactions. Recombinant SEPT9-containing octamers will make it possible to design cell-free assays to dissect the complex interactions of septins with cell membranes and the actin/microtubule cytoskeleton.

Summary Human septins in cells form hetero-octameric complexes containing the ubiquitously expressed SEPT9. Iv et al. describe the first-time isolation of recombinant human septin octamers with distinct SEPT9 isoforms. Reconstitution studies show that octamers with either a long or a short SEPT9 isoform form higher-order filament assemblies and directly bind and cross-link actin filaments.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted January 22, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms
Francois Iv, Carla Silva Martins, Gerard Castro-Linares, Cyntia Taveneau, Pascale Barbier, Pascal Verdier-Pinard, Luc Camoin, Stéphane Audebert, Feng-Ching Tsai, Laurie Ramond, Alex Llewellyn, Mayssa Belhabib, Koyomi Nakazawa, Aurélie Di Cicco, Renaud Vincentelli, Jerome Wenger, Stéphanie Cabantous, Gijsje H. Koenderink, Aurélie Bertin, Manos Mavrakis
bioRxiv 2021.01.21.427698; doi: https://doi.org/10.1101/2021.01.21.427698
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Insights into animal septins using recombinant human septin octamers with distinct SEPT9 isoforms
Francois Iv, Carla Silva Martins, Gerard Castro-Linares, Cyntia Taveneau, Pascale Barbier, Pascal Verdier-Pinard, Luc Camoin, Stéphane Audebert, Feng-Ching Tsai, Laurie Ramond, Alex Llewellyn, Mayssa Belhabib, Koyomi Nakazawa, Aurélie Di Cicco, Renaud Vincentelli, Jerome Wenger, Stéphanie Cabantous, Gijsje H. Koenderink, Aurélie Bertin, Manos Mavrakis
bioRxiv 2021.01.21.427698; doi: https://doi.org/10.1101/2021.01.21.427698

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Cell Biology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3589)
  • Biochemistry (7553)
  • Bioengineering (5498)
  • Bioinformatics (20742)
  • Biophysics (10305)
  • Cancer Biology (7962)
  • Cell Biology (11624)
  • Clinical Trials (138)
  • Developmental Biology (6596)
  • Ecology (10175)
  • Epidemiology (2065)
  • Evolutionary Biology (13586)
  • Genetics (9525)
  • Genomics (12824)
  • Immunology (7911)
  • Microbiology (19518)
  • Molecular Biology (7647)
  • Neuroscience (42014)
  • Paleontology (307)
  • Pathology (1254)
  • Pharmacology and Toxicology (2195)
  • Physiology (3260)
  • Plant Biology (7027)
  • Scientific Communication and Education (1294)
  • Synthetic Biology (1948)
  • Systems Biology (5420)
  • Zoology (1113)