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Immunogenic Potential of DNA Vaccine candidate, ZyCoV-D against SARS-CoV-2 in Animal Models

View ORCID ProfileAyan Dey, T.M. Chozhavel Rajanathan, Harish Chandra, Hari P.R. Pericherla, Sanjeev Kumar, Huzaifa S. Choonia, Mayank Bajpai, Arun K. Singh, Anuradha Sinha, Gurwinder Saini, Parth Dalal, Sarosh Vandriwala, Mohammed A. Raheem, Rupesh D. Divate, Neelam L. Navlani, Vibhuti Sharma, Aashini Parikh, Siva Prasath, Sankar Rao, Kapil Maithal
doi: https://doi.org/10.1101/2021.01.26.428240
Ayan Dey
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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T.M. Chozhavel Rajanathan
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Harish Chandra
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Hari P.R. Pericherla
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Sanjeev Kumar
2Zydus Research Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Huzaifa S. Choonia
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Mayank Bajpai
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Arun K. Singh
2Zydus Research Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Anuradha Sinha
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Gurwinder Saini
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Parth Dalal
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Sarosh Vandriwala
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Mohammed A. Raheem
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Rupesh D. Divate
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Neelam L. Navlani
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Vibhuti Sharma
2Zydus Research Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Aashini Parikh
2Zydus Research Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Siva Prasath
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Sankar Rao
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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Kapil Maithal
1Vaccine Technology Centre, Cadila Healthcare Ltd, Ahmedabad, India
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  • For correspondence: kapil.maithal@zyduscadila.com
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Abstract

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), initially originated in China in year 2019 and spread rapidly across the globe within 5 months, causing over 96 million cases of infection and over 2 million deaths. Huge efforts were undertaken to bring the COVID-19 vaccines in clinical development, so that it can be made available at the earliest, if found to be efficacious in the trials. We developed a candidate vaccine ZyCoV-D comprising of a DNA plasmid vector carrying the gene encoding the spike protein (S) of the SARS-CoV-2 virus. The S protein of the virus includes the receptor binding domain (RBD), responsible for binding to the human angiotensin converting enzyme (ACE-2) receptor. The DNA plasmid construct was transformed into E. coli cells for large scale production. The immunogenicity potential of the plasmid DNA has been evaluated in mice, guinea pig, and rabbit models by intradermal route at 25, 100 and 500μg dose. Based on the animal studies proof-of-concept has been established and preclinical toxicology (PCT) studies were conducted in rat and rabbit model. Preliminary animal study demonstrates that the candidate DNA vaccine induces antibody response including neutralizing antibodies against SARS-CoV-2 and also provided Th-1 response as evidenced by elevated IFN-γ levels.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted January 26, 2021.
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Immunogenic Potential of DNA Vaccine candidate, ZyCoV-D against SARS-CoV-2 in Animal Models
Ayan Dey, T.M. Chozhavel Rajanathan, Harish Chandra, Hari P.R. Pericherla, Sanjeev Kumar, Huzaifa S. Choonia, Mayank Bajpai, Arun K. Singh, Anuradha Sinha, Gurwinder Saini, Parth Dalal, Sarosh Vandriwala, Mohammed A. Raheem, Rupesh D. Divate, Neelam L. Navlani, Vibhuti Sharma, Aashini Parikh, Siva Prasath, Sankar Rao, Kapil Maithal
bioRxiv 2021.01.26.428240; doi: https://doi.org/10.1101/2021.01.26.428240
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Immunogenic Potential of DNA Vaccine candidate, ZyCoV-D against SARS-CoV-2 in Animal Models
Ayan Dey, T.M. Chozhavel Rajanathan, Harish Chandra, Hari P.R. Pericherla, Sanjeev Kumar, Huzaifa S. Choonia, Mayank Bajpai, Arun K. Singh, Anuradha Sinha, Gurwinder Saini, Parth Dalal, Sarosh Vandriwala, Mohammed A. Raheem, Rupesh D. Divate, Neelam L. Navlani, Vibhuti Sharma, Aashini Parikh, Siva Prasath, Sankar Rao, Kapil Maithal
bioRxiv 2021.01.26.428240; doi: https://doi.org/10.1101/2021.01.26.428240

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