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Neutralization of SARS-CoV-2 spike 69/70 deletion, E484K, and N501Y variants by BNT162b2 vaccine-elicited sera

Xuping Xie, Yang Liu, Jianying Liu, Xianwen Zhang, Jing Zou, Camila R. Fontes-Garfias, Hongjie Xia, Kena A. Swanson, Mark Cutler, David Cooper, Vineet D. Menachery, Scott Weaver, Philip R. Dormitzer, Pei-Yong Shi
doi: https://doi.org/10.1101/2021.01.27.427998
Xuping Xie
1Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston TX, U.S.A.
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Yang Liu
1Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston TX, U.S.A.
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Jianying Liu
2Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston TX, U.S.A.
3Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, TX, U.S.A.
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Xianwen Zhang
1Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston TX, U.S.A.
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Jing Zou
1Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston TX, U.S.A.
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Camila R. Fontes-Garfias
1Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston TX, U.S.A.
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Hongjie Xia
1Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston TX, U.S.A.
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Kena A. Swanson
4Pfizer, 401 N Middletown Rd., Pearl River, NY 10960, U.S.A.
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Mark Cutler
4Pfizer, 401 N Middletown Rd., Pearl River, NY 10960, U.S.A.
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David Cooper
4Pfizer, 401 N Middletown Rd., Pearl River, NY 10960, U.S.A.
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Vineet D. Menachery
2Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston TX, U.S.A.
3Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, TX, U.S.A.
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Scott Weaver
2Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston TX, U.S.A.
3Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, TX, U.S.A.
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Philip R. Dormitzer
4Pfizer, 401 N Middletown Rd., Pearl River, NY 10960, U.S.A.
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  • For correspondence: Philip.Dormitzer@pfizer.com peshi@UTMB.edu
Pei-Yong Shi
1Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston TX, U.S.A.
3Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, TX, U.S.A.
5Institute for Translational Sciences, University of Texas Medical Branch, Galveston, TX, U.S.A.
6Sealy Institute for Vaccine Sciences, University of Texas Medical Branch, Galveston, TX, U.S.A.
7Sealy Center for Structural Biology & Molecular Biophysics, University of Texas Medical Branch, Galveston, TX, U.S.A.
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  • For correspondence: Philip.Dormitzer@pfizer.com peshi@UTMB.edu
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Abstract

We engineered three SARS-CoV-2 viruses containing key spike mutations from the newly emerged United Kingdom (UK) and South African (SA) variants: N501Y from UK and SA; 69/70-deletion+N501Y+D614G from UK; and E484K+N501Y+D614G from SA. Neutralization geometric mean titers (GMTs) of twenty BTN162b2 vaccine-elicited human sera against the three mutant viruses were 0.81- to 1.46-fold of the GMTs against parental virus, indicating small effects of these mutations on neutralization by sera elicited by two BNT162b2 doses.

Competing Interest Statement

X.X., V.D.M., and P.-Y.S. have filed a patent on the reverse genetic system. K.A.S., M.C., D.C., and P.R.D. are employees of Pfizer and may hold stock options. X.X., J.Z., C.R.F.G., H.X., and P.-Y.S. received compensation from Pfizer to perform the neutralization assay. Other authors declare no competing interests.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted January 27, 2021.
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Neutralization of SARS-CoV-2 spike 69/70 deletion, E484K, and N501Y variants by BNT162b2 vaccine-elicited sera
Xuping Xie, Yang Liu, Jianying Liu, Xianwen Zhang, Jing Zou, Camila R. Fontes-Garfias, Hongjie Xia, Kena A. Swanson, Mark Cutler, David Cooper, Vineet D. Menachery, Scott Weaver, Philip R. Dormitzer, Pei-Yong Shi
bioRxiv 2021.01.27.427998; doi: https://doi.org/10.1101/2021.01.27.427998
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Neutralization of SARS-CoV-2 spike 69/70 deletion, E484K, and N501Y variants by BNT162b2 vaccine-elicited sera
Xuping Xie, Yang Liu, Jianying Liu, Xianwen Zhang, Jing Zou, Camila R. Fontes-Garfias, Hongjie Xia, Kena A. Swanson, Mark Cutler, David Cooper, Vineet D. Menachery, Scott Weaver, Philip R. Dormitzer, Pei-Yong Shi
bioRxiv 2021.01.27.427998; doi: https://doi.org/10.1101/2021.01.27.427998

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