Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Heterologous vaccination regimens with self-amplifying RNA and Adenoviral COVID vaccines induce superior immune responses than single dose vaccine regimens in mice

View ORCID ProfileAlexandra J Spencer, View ORCID ProfilePaul F McKay, View ORCID ProfileSandra Belij-Rammerstorfer, View ORCID ProfileMarta Ulaszewska, View ORCID ProfileCameron D Bissett, Kai Hu, Karnyart Samnuan, View ORCID ProfileDaniel Wright, View ORCID ProfileHannah R Sharpe, View ORCID ProfileCiaran Gilbride, Adam Truby, View ORCID ProfileElizabeth R Allen, View ORCID ProfileSarah C Gilbert, View ORCID ProfileRobin J Shattock, View ORCID ProfileTeresa Lambe
doi: https://doi.org/10.1101/2021.01.28.428665
Alexandra J Spencer
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Alexandra J Spencer
  • For correspondence: alex.spencer@ndm.ox.ac.uk
Paul F McKay
2Department of Infectious Disease, Imperial College London, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Paul F McKay
Sandra Belij-Rammerstorfer
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sandra Belij-Rammerstorfer
Marta Ulaszewska
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Marta Ulaszewska
Cameron D Bissett
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Cameron D Bissett
Kai Hu
2Department of Infectious Disease, Imperial College London, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Karnyart Samnuan
2Department of Infectious Disease, Imperial College London, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Daniel Wright
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Daniel Wright
Hannah R Sharpe
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Hannah R Sharpe
Ciaran Gilbride
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Ciaran Gilbride
Adam Truby
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Elizabeth R Allen
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Elizabeth R Allen
Sarah C Gilbert
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Sarah C Gilbert
Robin J Shattock
2Department of Infectious Disease, Imperial College London, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Robin J Shattock
Teresa Lambe
1The Jenner Institute, Nuffield Department of Medicine, University of Oxford, United Kingdom
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Teresa Lambe
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Several vaccines have demonstrated efficacy against SARS-CoV-2 mediated disease, yet there is limited data on the immune response induced by heterologous vaccination regimens using alternate vaccine modalities. Here, we present a detailed description of the immune response, in mice, following vaccination with a self-amplifying RNA (saRNA) vaccine and an adenoviral vectored vaccine (ChAdOx1 nCoV-19/AZD1222) against SARS-CoV-2. We demonstrate that antibody responses are higher in two dose heterologous vaccination regimens than single dose regimens, with high titre neutralising antibodies induced. Importantly, the cellular immune response after a heterologous regimen is dominated by cytotoxic T cells and Th1+ CD4 T cells which is superior to the response induced in homologous vaccination regimens in mice.

Competing Interest Statement

SCG is co-founder and board member of Vaccitech (collaborators in the early development of this vaccine candidate) and named as an inventor on a patent covering use of ChAdOx1-vectored vaccines and a patent application covering this SARS-CoV-2 vaccine. TL is named as an inventor on a patent application covering this SARS-CoV-2 vaccine and was consultant to Vaccitech. PMK and RJS are co-founders and RJS is a board member of VaxEquity and VacEquity and are named inventors on a patent application covering the SARS-CoV-2 saRNA vaccine candidate.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted January 29, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Heterologous vaccination regimens with self-amplifying RNA and Adenoviral COVID vaccines induce superior immune responses than single dose vaccine regimens in mice
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Heterologous vaccination regimens with self-amplifying RNA and Adenoviral COVID vaccines induce superior immune responses than single dose vaccine regimens in mice
Alexandra J Spencer, Paul F McKay, Sandra Belij-Rammerstorfer, Marta Ulaszewska, Cameron D Bissett, Kai Hu, Karnyart Samnuan, Daniel Wright, Hannah R Sharpe, Ciaran Gilbride, Adam Truby, Elizabeth R Allen, Sarah C Gilbert, Robin J Shattock, Teresa Lambe
bioRxiv 2021.01.28.428665; doi: https://doi.org/10.1101/2021.01.28.428665
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Heterologous vaccination regimens with self-amplifying RNA and Adenoviral COVID vaccines induce superior immune responses than single dose vaccine regimens in mice
Alexandra J Spencer, Paul F McKay, Sandra Belij-Rammerstorfer, Marta Ulaszewska, Cameron D Bissett, Kai Hu, Karnyart Samnuan, Daniel Wright, Hannah R Sharpe, Ciaran Gilbride, Adam Truby, Elizabeth R Allen, Sarah C Gilbert, Robin J Shattock, Teresa Lambe
bioRxiv 2021.01.28.428665; doi: https://doi.org/10.1101/2021.01.28.428665

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Immunology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4232)
  • Biochemistry (9128)
  • Bioengineering (6774)
  • Bioinformatics (23989)
  • Biophysics (12117)
  • Cancer Biology (9523)
  • Cell Biology (13772)
  • Clinical Trials (138)
  • Developmental Biology (7627)
  • Ecology (11686)
  • Epidemiology (2066)
  • Evolutionary Biology (15504)
  • Genetics (10638)
  • Genomics (14322)
  • Immunology (9477)
  • Microbiology (22832)
  • Molecular Biology (9089)
  • Neuroscience (48957)
  • Paleontology (355)
  • Pathology (1480)
  • Pharmacology and Toxicology (2568)
  • Physiology (3844)
  • Plant Biology (8327)
  • Scientific Communication and Education (1471)
  • Synthetic Biology (2296)
  • Systems Biology (6186)
  • Zoology (1300)