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History
- January 29, 2021.
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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Author Information
- André D. G. Leitão1,+,
- Paulina Rudolffi Soto1,
- Alexandre Chappard1,3,
- Akshay Bhumkar1,2,
- Dominic J. B. Hunter4,
- Emma Sierecki1,* and
- Yann Gambin1,*
- 1EMBL Australia Node in Single Molecule Science and School of Medical Sciences, The University of New South Wales, Sydney, NSW 2031, Australia
- 2Woolcock Institute of Medical Research, University of Sydney, Sydney, NSW 2037
- 3School of Chemistry, The University of Edinburgh, Edinburgh, EH9 3FJ, United Kingdom
- 4Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD 4072, Australia
- ↵*To whom correspondence should be addressed: Yann Gambin, y.gambin{at}unsw.edu.au and to Emma Sierecki, e.sierecki{at}unsw.edu.au.
Author Contributions: A.D.G.L., Y.G. and E.S. designed the research. A.D.G.L. performed cell-free protein expressions and co-expressions; purification and labelling of α-Synuclein; production of pre-formed fibrils of α-Synuclein and two-color coincidence experiments. D.J.B.H. performed gateway cloning of the library of Lewy Body proteins constructs. P.R.S. and A.B. performed AlphaScreen experiments. A.B. helped with purification of α-Synuclein. A.C. helped with construct preparation and AlphaScreen assays. D.J.B.H. produced all cell-free lysates used in this study. Y.G. and E.S. directed the research. A.D.G.L., Y.G. and E.S. wrote the manuscript and all authors contributed to the revisions of the manuscript.