Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

The SARS-CoV-2 Y453F mink variant displays a striking increase in ACE-2 affinity but does not challenge antibody neutralization

Rafael Bayarri-Olmos, Anne Rosbjerg, Laust Bruun Johnsen, Charlotte Helgstrand, Theresa Bak-Thomsen, Peter Garred, View ORCID ProfileMikkel-Ole Skjoedt
doi: https://doi.org/10.1101/2021.01.29.428834
Rafael Bayarri-Olmos
1Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Anne Rosbjerg
1Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Laust Bruun Johnsen
2Novo Nordisk A/S, Måløv, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Charlotte Helgstrand
2Novo Nordisk A/S, Måløv, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Theresa Bak-Thomsen
2Novo Nordisk A/S, Måløv, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Peter Garred
1Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Mikkel-Ole Skjoedt
1Rigshospitalet, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark
3Institute of Immunology and Microbiology, University of Copenhagen
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Mikkel-Ole Skjoedt
  • For correspondence: moskjoedt@sund.ku.dk
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

SARS-CoV-2 transmission from humans to animals has been reported for many domesticated species, including cats, dogs and minks. Identification of novel spike gene mutations appearing in minks has raised major concerns about potential immune evasion and challenges for the global vaccine strategy. The genetic variant, known as “cluster-five”, arose among farmed minks in Denmark and resulted in a complete shutdown of the world’s largest mink production. However, the functional properties of this new variant are not established. Here we present functional data on the Y453F cluster-five receptor-binding domain (RBD) and show that it does not decrease established humoral immunity or affect the neutralizing response in a vaccine model based on wild-type RBD or spike. However, it binds the human ACE-2 receptor with a four-fold higher affinity suggesting an enhanced transmission capacity and a possible challenge for viral control.

  • Abbreviations
    RBD
    Receptor-binding domain
    ACE-2
    Angiotensin-converting enzyme 2
    BLI
    Biolayer interferometry
  • Copyright 
    The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
    Back to top
    PreviousNext
    Posted January 29, 2021.
    Download PDF
    Email

    Thank you for your interest in spreading the word about bioRxiv.

    NOTE: Your email address is requested solely to identify you as the sender of this article.

    Enter multiple addresses on separate lines or separate them with commas.
    The SARS-CoV-2 Y453F mink variant displays a striking increase in ACE-2 affinity but does not challenge antibody neutralization
    (Your Name) has forwarded a page to you from bioRxiv
    (Your Name) thought you would like to see this page from the bioRxiv website.
    CAPTCHA
    This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
    Share
    The SARS-CoV-2 Y453F mink variant displays a striking increase in ACE-2 affinity but does not challenge antibody neutralization
    Rafael Bayarri-Olmos, Anne Rosbjerg, Laust Bruun Johnsen, Charlotte Helgstrand, Theresa Bak-Thomsen, Peter Garred, Mikkel-Ole Skjoedt
    bioRxiv 2021.01.29.428834; doi: https://doi.org/10.1101/2021.01.29.428834
    Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
    Citation Tools
    The SARS-CoV-2 Y453F mink variant displays a striking increase in ACE-2 affinity but does not challenge antibody neutralization
    Rafael Bayarri-Olmos, Anne Rosbjerg, Laust Bruun Johnsen, Charlotte Helgstrand, Theresa Bak-Thomsen, Peter Garred, Mikkel-Ole Skjoedt
    bioRxiv 2021.01.29.428834; doi: https://doi.org/10.1101/2021.01.29.428834

    Citation Manager Formats

    • BibTeX
    • Bookends
    • EasyBib
    • EndNote (tagged)
    • EndNote 8 (xml)
    • Medlars
    • Mendeley
    • Papers
    • RefWorks Tagged
    • Ref Manager
    • RIS
    • Zotero
    • Tweet Widget
    • Facebook Like
    • Google Plus One

    Subject Area

    • Immunology
    Subject Areas
    All Articles
    • Animal Behavior and Cognition (3607)
    • Biochemistry (7581)
    • Bioengineering (5529)
    • Bioinformatics (20809)
    • Biophysics (10338)
    • Cancer Biology (7988)
    • Cell Biology (11647)
    • Clinical Trials (138)
    • Developmental Biology (6611)
    • Ecology (10217)
    • Epidemiology (2065)
    • Evolutionary Biology (13630)
    • Genetics (9550)
    • Genomics (12854)
    • Immunology (7925)
    • Microbiology (19555)
    • Molecular Biology (7668)
    • Neuroscience (42147)
    • Paleontology (308)
    • Pathology (1258)
    • Pharmacology and Toxicology (2203)
    • Physiology (3269)
    • Plant Biology (7051)
    • Scientific Communication and Education (1294)
    • Synthetic Biology (1952)
    • Systems Biology (5429)
    • Zoology (1119)