Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Nitroxoline as a promising alternative drug for the treatment of Lyme disease based on an in-vitro study

View ORCID ProfileHector S. Alvarez-Manzo, View ORCID ProfileYumin Zhang, Wanliang Shi, Ying Zhang
doi: https://doi.org/10.1101/2021.02.04.429852
Hector S. Alvarez-Manzo
1Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA; (H.S.A.-M.); (YM.Z.); (W.S.); (H.S.A.-M.); (YM.Z.); (W.S.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Hector S. Alvarez-Manzo
  • For correspondence: halvare3@jhu.edu yzhan424@jhmi.edu wshi3@jhu.edu halvare3@jhu.edu yzhan424@jhmi.edu wshi3@jhu.edu
Yumin Zhang
1Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA; (H.S.A.-M.); (YM.Z.); (W.S.); (H.S.A.-M.); (YM.Z.); (W.S.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • ORCID record for Yumin Zhang
  • For correspondence: halvare3@jhu.edu yzhan424@jhmi.edu wshi3@jhu.edu halvare3@jhu.edu yzhan424@jhmi.edu wshi3@jhu.edu
Wanliang Shi
1Department of Molecular Microbiology and Immunology, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA; (H.S.A.-M.); (YM.Z.); (W.S.); (H.S.A.-M.); (YM.Z.); (W.S.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: halvare3@jhu.edu yzhan424@jhmi.edu wshi3@jhu.edu halvare3@jhu.edu yzhan424@jhmi.edu wshi3@jhu.edu
Ying Zhang
2State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310003, China
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: yzhang207@zju.edu.cn
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Preview PDF
Loading

Abstract

Lyme disease (LD) is the most common vector-borne disease in USA and Europe and is caused by Borrelia burgdorferi. Despite proper treatment, approximately one fifth of patients will develop post-treatment LD syndrome (PTLDS), a condition which is poorly understood. One of the possible causes is thought to be due to persister forms of B. burgdorferi that are not effectively killed by the current Lyme antibiotics. In this study, we evaluated nitroxoline, an antibiotic used to treat urinary tract infections, for its activity against a stationary-phase culture enriched with persister forms of B. burgdorferi. Nitroxoline was found to be equivalent in activity against B. burgdorferi to cefuroxime (standard Lyme antibiotic) in different experiments. Moreover, we found that the three-drug combination cefuroxime + nitroxoline + clarithromycin eradicated 98.3% of stationary phase bacteria in the drug-exposure experiment and prevented the regrowth in the subculture study after drug exposure, as well as two-drug combinations cefuroxime + nitroxoline and clarithromycin + nitroxoline. These drug combinations should be further evaluated in a LD mouse model to assess if eradication of persister forms of B. burgdorferi in-vivo is possible and if so, whether nitroxoline could be repurposed as an alternative drug for the treatment of LD.

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
Back to top
PreviousNext
Posted February 05, 2021.
Download PDF
Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Nitroxoline as a promising alternative drug for the treatment of Lyme disease based on an in-vitro study
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Nitroxoline as a promising alternative drug for the treatment of Lyme disease based on an in-vitro study
Hector S. Alvarez-Manzo, Yumin Zhang, Wanliang Shi, Ying Zhang
bioRxiv 2021.02.04.429852; doi: https://doi.org/10.1101/2021.02.04.429852
Reddit logo Twitter logo Facebook logo LinkedIn logo Mendeley logo
Citation Tools
Nitroxoline as a promising alternative drug for the treatment of Lyme disease based on an in-vitro study
Hector S. Alvarez-Manzo, Yumin Zhang, Wanliang Shi, Ying Zhang
bioRxiv 2021.02.04.429852; doi: https://doi.org/10.1101/2021.02.04.429852

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Microbiology
Subject Areas
All Articles
  • Animal Behavior and Cognition (4658)
  • Biochemistry (10312)
  • Bioengineering (7633)
  • Bioinformatics (26237)
  • Biophysics (13472)
  • Cancer Biology (10647)
  • Cell Biology (15359)
  • Clinical Trials (138)
  • Developmental Biology (8462)
  • Ecology (12775)
  • Epidemiology (2067)
  • Evolutionary Biology (16789)
  • Genetics (11370)
  • Genomics (15427)
  • Immunology (10575)
  • Microbiology (25083)
  • Molecular Biology (10171)
  • Neuroscience (54229)
  • Paleontology (398)
  • Pathology (1660)
  • Pharmacology and Toxicology (2883)
  • Physiology (4324)
  • Plant Biology (9207)
  • Scientific Communication and Education (1582)
  • Synthetic Biology (2543)
  • Systems Biology (6760)
  • Zoology (1457)