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Self-assembling SARS-CoV-2 nanoparticle vaccines targeting the S protein induces protective immunity in mice

View ORCID ProfileXingjian Liu, Haozhi Song, Jianmin Jiang, Xintao Gao, Yongzhu Yi, Yuting Shang, Jialei Li, Dan Li, Zhen Zeng, Yinü Li, Zhifang Zhang
doi: https://doi.org/10.1101/2021.02.05.428685
Xingjian Liu
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
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Haozhi Song
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
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Jianmin Jiang
2Key Laboratory of Vaccine, Prevention and control of Infectious disease of Zhejiang Province, Zhejiang Provincial Center For Disease Control And Prevention, Hangzhou, Zhejiang Province, China
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Xintao Gao
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
3Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
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Yongzhu Yi
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
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Yuting Shang
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
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Jialei Li
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
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Dan Li
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
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Zhen Zeng
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
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Yinü Li
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
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  • For correspondence: liyinv@caas.cn bri-zhangzhifang@caas.cn
Zhifang Zhang
1Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China
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  • For correspondence: liyinv@caas.cn bri-zhangzhifang@caas.cn
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Abstract

The spike (S), a homotrimer glycoprotein, is the most important antigen target in the research and development of SARS-CoV-2 vaccine. There is no doubt that fully simulating the advanced structure of this homotrimer in the subunit vaccine development strategy is the most likely way to improve the immune protective effect of the vaccine. In this study, the preparation strategies of S protein receptor-binding domain (RBD) trimer, S1 region trimer, and ectodomain (ECD) trimer nanoparticles were designed based on ferritin nanoparticle self-assembly technology. The Bombyx mori baculovirus expression system was used to prepare these three nanoparticle vaccines with high expression levels in the silkworm. The immune results of mice show that the nanoparticle vaccine prepared by this strategy can not only induce an immune response by subcutaneous administration but also effective by oral administration. Given the stability of these ferritin-based nanoparticles vaccine, easy-to-use and low-cost oral immunization strategy can make up for the vaccination blind areas caused by the shortage of ultralow-temperature equipment and medical resources in underdeveloped areas. And the oral vaccine is also a very potential candidate to cut off the spread of SARS-CoV-2 in domestic and farmed animals, especially in stray and wild animals.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted February 07, 2021.
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Self-assembling SARS-CoV-2 nanoparticle vaccines targeting the S protein induces protective immunity in mice
Xingjian Liu, Haozhi Song, Jianmin Jiang, Xintao Gao, Yongzhu Yi, Yuting Shang, Jialei Li, Dan Li, Zhen Zeng, Yinü Li, Zhifang Zhang
bioRxiv 2021.02.05.428685; doi: https://doi.org/10.1101/2021.02.05.428685
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Self-assembling SARS-CoV-2 nanoparticle vaccines targeting the S protein induces protective immunity in mice
Xingjian Liu, Haozhi Song, Jianmin Jiang, Xintao Gao, Yongzhu Yi, Yuting Shang, Jialei Li, Dan Li, Zhen Zeng, Yinü Li, Zhifang Zhang
bioRxiv 2021.02.05.428685; doi: https://doi.org/10.1101/2021.02.05.428685

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