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Alternative architecture of the E. coli chemosensory array

View ORCID ProfileAlister Burt, C. Keith Cassidy, View ORCID ProfilePhillip J. Stansfeld, View ORCID ProfileIrina Gutsche
doi: https://doi.org/10.1101/2021.02.05.429929
Alister Burt
1Institut de Biologie Structurale, Univ. Grenoble Alpes, CEA, CNRS, IBS, 71 Avenue des martyrs, F-38044 Grenoble, France
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C. Keith Cassidy
2Department of Biochemistry, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK
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Phillip J. Stansfeld
3School of Life Sciences & Department of Chemistry, University of Warwick, Gibbet Hill Campus, CV4 7AL, UK
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Irina Gutsche
1Institut de Biologie Structurale, Univ. Grenoble Alpes, CEA, CNRS, IBS, 71 Avenue des martyrs, F-38044 Grenoble, France
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  • For correspondence: irina.gutsche@ibs.fr
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Abstract

Chemotactic responses in motile bacteria are the result of sophisticated signal transduction by large, highly organized arrays of sensory proteins. Despite tremendous progress in the understanding of chemosensory array structure and function, a structural basis for the heightened sensitivity of networked chemoreceptors is not yet complete. Here we present cryo-electron tomography visualisations of native-state chemosensory arrays in E. coli minicells. Strikingly, these arrays exhibit a p2-symmetric array architecture that differs markedly from the p6-symmetric architecture previously described in E. coli. Based on this data, we propose molecular models of this alternative architecture and the canonical p6-symmetric assembly. We evaluate our observations and each model in the context of previously published data, assessing the functional implications of an alternative architecture and effects for future studies.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted February 05, 2021.
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Alternative architecture of the E. coli chemosensory array
Alister Burt, C. Keith Cassidy, Phillip J. Stansfeld, Irina Gutsche
bioRxiv 2021.02.05.429929; doi: https://doi.org/10.1101/2021.02.05.429929
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Alternative architecture of the E. coli chemosensory array
Alister Burt, C. Keith Cassidy, Phillip J. Stansfeld, Irina Gutsche
bioRxiv 2021.02.05.429929; doi: https://doi.org/10.1101/2021.02.05.429929

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