Skip to main content
bioRxiv
  • Home
  • About
  • Submit
  • ALERTS / RSS
Advanced Search
New Results

Murine ex vivo cultured alveolar macrophages provide a novel tool to study tissue-resident macrophage behavior and function

A.-D. Gorki, D. Symmank, S. Zahalka, K. Lakovits, A. Hladik, B. Langer, B. Maurer, V. Sexl, R. Kain, S. Knapp
doi: https://doi.org/10.1101/2021.02.11.430791
A.-D. Gorki
1Research Laboratory of Infection Biology, Department of Medicine I, Medical University of Vienna, Austria
2CeMM, Research Center for Molecular Medicine of the Austrian Academy of Sciences, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
D. Symmank
1Research Laboratory of Infection Biology, Department of Medicine I, Medical University of Vienna, Austria
2CeMM, Research Center for Molecular Medicine of the Austrian Academy of Sciences, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
S. Zahalka
1Research Laboratory of Infection Biology, Department of Medicine I, Medical University of Vienna, Austria
2CeMM, Research Center for Molecular Medicine of the Austrian Academy of Sciences, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
K. Lakovits
1Research Laboratory of Infection Biology, Department of Medicine I, Medical University of Vienna, Austria
2CeMM, Research Center for Molecular Medicine of the Austrian Academy of Sciences, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
A. Hladik
1Research Laboratory of Infection Biology, Department of Medicine I, Medical University of Vienna, Austria
2CeMM, Research Center for Molecular Medicine of the Austrian Academy of Sciences, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
B. Langer
3Department of Pathology, Medical University of Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
B. Maurer
4Institute of Pharmacology and Toxicology, University of Veterinary Medicine, Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
V. Sexl
4Institute of Pharmacology and Toxicology, University of Veterinary Medicine, Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
R. Kain
3Department of Pathology, Medical University of Vienna, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
S. Knapp
1Research Laboratory of Infection Biology, Department of Medicine I, Medical University of Vienna, Austria
2CeMM, Research Center for Molecular Medicine of the Austrian Academy of Sciences, Austria
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • For correspondence: sylvia.knapp@meduniwien.ac.at
  • Abstract
  • Full Text
  • Info/History
  • Metrics
  • Supplementary material
  • Preview PDF
Loading

SUMMARY

Tissue-resident macrophages are of vital importance as they preserve tissue homeostasis in all mammalian organs. Nevertheless, appropriate cell culture models are still limited. Here, we propose a novel culture model to study and expand murine primary alveolar macrophages (AMs), the tissue-resident macrophages of the lung, in vitro over several months. By providing a combination of GM-CSF, TGFβ and the PPARγ activator rosiglitazone, we maintain and expand mouse ex vivo cultured AMs, short mexAMs, over several months. MexAMs maintain typical morphologic features and stably express primary AM surface markers throughout in vitro culture. They respond to microbial ligands and exhibit an AM-like transcriptional profile, including the expression of AM specific transcription factors. Furthermore, when transferred into AM deficient mice, mexAMs efficiently engraft in the lung and fulfill key macrophage functions leading to a significantly reduced surfactant load in those mice. Altogether, mexAMs provide a novel, simple and versatile tool to study AM behavior in homeostasis and disease settings.

KEYPOINTS

  • A novel method to culture and expand primary alveolar macrophages over several months ex vivo

  • Murine ex vivo cultured alveolar macrophages (mexAMs) restore lung function in a murine pulmonary alveolar proteinosis model

Competing Interest Statement

The authors have declared no competing interest.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
Back to top
PreviousNext
Posted February 11, 2021.
Download PDF

Supplementary Material

Email

Thank you for your interest in spreading the word about bioRxiv.

NOTE: Your email address is requested solely to identify you as the sender of this article.

Enter multiple addresses on separate lines or separate them with commas.
Murine ex vivo cultured alveolar macrophages provide a novel tool to study tissue-resident macrophage behavior and function
(Your Name) has forwarded a page to you from bioRxiv
(Your Name) thought you would like to see this page from the bioRxiv website.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Share
Murine ex vivo cultured alveolar macrophages provide a novel tool to study tissue-resident macrophage behavior and function
A.-D. Gorki, D. Symmank, S. Zahalka, K. Lakovits, A. Hladik, B. Langer, B. Maurer, V. Sexl, R. Kain, S. Knapp
bioRxiv 2021.02.11.430791; doi: https://doi.org/10.1101/2021.02.11.430791
Digg logo Reddit logo Twitter logo Facebook logo Google logo LinkedIn logo Mendeley logo
Citation Tools
Murine ex vivo cultured alveolar macrophages provide a novel tool to study tissue-resident macrophage behavior and function
A.-D. Gorki, D. Symmank, S. Zahalka, K. Lakovits, A. Hladik, B. Langer, B. Maurer, V. Sexl, R. Kain, S. Knapp
bioRxiv 2021.02.11.430791; doi: https://doi.org/10.1101/2021.02.11.430791

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Subject Area

  • Immunology
Subject Areas
All Articles
  • Animal Behavior and Cognition (3607)
  • Biochemistry (7581)
  • Bioengineering (5529)
  • Bioinformatics (20809)
  • Biophysics (10338)
  • Cancer Biology (7988)
  • Cell Biology (11647)
  • Clinical Trials (138)
  • Developmental Biology (6611)
  • Ecology (10217)
  • Epidemiology (2065)
  • Evolutionary Biology (13630)
  • Genetics (9550)
  • Genomics (12854)
  • Immunology (7925)
  • Microbiology (19555)
  • Molecular Biology (7668)
  • Neuroscience (42147)
  • Paleontology (308)
  • Pathology (1258)
  • Pharmacology and Toxicology (2203)
  • Physiology (3269)
  • Plant Biology (7051)
  • Scientific Communication and Education (1294)
  • Synthetic Biology (1952)
  • Systems Biology (5429)
  • Zoology (1119)