Abstract
Protein kinases exhibit significant structural diversity, primarily in the conformation of the activation loop and other components of the active site. We previously performed a clustering of the conformation of the activation loop of all protein kinase structures in the Protein Data Bank (Modi and Dunbrack, PNAS, 116:6818-6827, 2019) into 8 classes based on the location of the Phe side chain of the DFG motif at the N-terminus of the activation loop. This is determined with a distance metric that measures the difference in the dihedral angles that determine the placement of the Phe side chains (the ϕ, ψ of X, D, and F of the X-DFG motif and the χ1 of the Phe side chain). The nomenclature is based on the regions of the Ramachandran map occupied by the XDF residues and the χ1 rotamer of the Phe residue. All active structures are “BLAminus”, while common inactive DFGin conformations are “BLBplus” and “ABAminus”. Type II inhibitors bind almost exclusively to the DFGout “BBAminus” conformation. In this paper, we present Kincore (http://dunbrack.fccc.edu/kincore), a web resource providing access to the conformational assignments based on our clustering along with labels for ligand types (Type I, Type II, etc.) bound to each kinase chain in the PDB. The data are annotated with several properties including PDBid, Uniprotid, gene, protein name, phylogenetic group, spatial and dihedral labels for orientation of DFGmotif residues, C-helix disposition, ligand name and type. The user can browse and query the database using these attributes individually or perform advanced search using a combination of them like a phylogenetic group with specific conformational label and ligand type. The user can also determine the spatial and dihedral labels for a structure with unknown conformation using the web server and standalone program. The entire database can be downloaded as text files and structure files in PyMOL sessions and mmCIF format. We believe that Kincore will help in understanding conformational dynamics of these proteins and guide development of inhibitors targeting specific states.
Competing Interest Statement
The authors have declared no competing interest.