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MEMS enabled miniaturized light-sheet microscopy with all optical control

Spyridon Bakas, Deepak Uttamchandani, Hiroshi Toshiyoshi, Ralf Bauer
doi: https://doi.org/10.1101/2021.02.13.431066
Spyridon Bakas
1Department of Electronic and Electrical Engineering, University of Strathclyde, Glasgow, UK
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  • For correspondence: Spyridon.Bakas@strath.ac.uk
Deepak Uttamchandani
1Department of Electronic and Electrical Engineering, University of Strathclyde, Glasgow, UK
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Hiroshi Toshiyoshi
2Institute of Industrial Science, University of Tokyo, Tokyo, Japan
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Ralf Bauer
1Department of Electronic and Electrical Engineering, University of Strathclyde, Glasgow, UK
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  • For correspondence: Ralf.Bauer@strath.ac.uk
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Abstract

We have designed and implemented a compact, cost-efficient miniaturised light-sheet microscopy system based on optical Microelectromechanical Systems (MEMS) scanners and tunable lenses. The system occupies a footprint of 20 × 28 × 13 cm3 and combines off-the-shelf optics and optomechanics with 3D-printed structural and optical elements, and an economically costed objective lens, excitation laser and camera. All-optical volume scanning enables imaging of 340 × 190 × 60 µm3 volumes with 0.25 vps and minimum lateral and axial resolution of 0.9 µm and 2.95 µm respectively. An open-top geometry allows imaging of samples on flat bottomed holders, allowing integration with microfluidic devices, multi-well plates and slide mounted samples, with applications envisaged in biomedical research and pre-clinical settings.

Competing Interest Statement

The authors have declared no competing interest.

Footnotes

  • Figure 2, SEM image changed to show better overview of MEMS device.

Copyright 
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license.
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Posted February 16, 2021.
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MEMS enabled miniaturized light-sheet microscopy with all optical control
Spyridon Bakas, Deepak Uttamchandani, Hiroshi Toshiyoshi, Ralf Bauer
bioRxiv 2021.02.13.431066; doi: https://doi.org/10.1101/2021.02.13.431066
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MEMS enabled miniaturized light-sheet microscopy with all optical control
Spyridon Bakas, Deepak Uttamchandani, Hiroshi Toshiyoshi, Ralf Bauer
bioRxiv 2021.02.13.431066; doi: https://doi.org/10.1101/2021.02.13.431066

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