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Nanoscale resolution of microbial fiber degradation in action

View ORCID ProfileMeltem Tatlı, View ORCID ProfileSarah Moraïs, View ORCID ProfileOmar E. Tovar-Herrera, View ORCID ProfileYannick Bomble, View ORCID ProfileEdward A. Bayer, View ORCID ProfileOhad Medalia, View ORCID ProfileItzhak Mizrahi
doi: https://doi.org/10.1101/2021.02.16.431430
Meltem Tatlı
1Department of Biochemistry, University of Zurich, Zurich, CH-8052, Switzerland
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Sarah Moraïs
2Faculty of Natural Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8499000 Israel
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Omar E. Tovar-Herrera
2Faculty of Natural Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8499000 Israel
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Yannick Bomble
3Biosciences Center, National Renewable Energy Laboratory, Golden, CO USA
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Edward A. Bayer
2Faculty of Natural Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8499000 Israel
4Department of Biomolecular Sciences, The Weizmann Institute of Science, Rehovot 7610001 Israel
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Ohad Medalia
1Department of Biochemistry, University of Zurich, Zurich, CH-8052, Switzerland
2Faculty of Natural Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8499000 Israel
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  • For correspondence: omedalia@bioc.uzh.ch imizrahi@bgu.ac.il
Itzhak Mizrahi
2Faculty of Natural Sciences, Ben-Gurion University of the Negev, Beer-Sheva 8499000 Israel
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  • For correspondence: omedalia@bioc.uzh.ch imizrahi@bgu.ac.il
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Abstract

Deconstruction of plant cell walls is imperative to global carbon cycling and sustainability efforts. Selected microbes degrade plant fibers using extremely efficient multi-enzymatic cellulosomes assemblies. Organization of cellulosomes on the bacterial cell surface and their ecological regulation remain elusive. By combining structural methodologies with molecular and biochemical approaches on the canonical Clostridium thermocellum system, we provide an unprecedented view into the in-situ structure and distribution of cellulosomal enzymes while interacting with their cellulosic substrate during fiber degradation. Structural exploration of growing cultures revealed isogenic phenotypic heterogeneity of cellulosome organization on single cells across the bacterial population, suggesting a division-of labor strategy driven by product-dependent dynamics. This study demonstrates how structural biology under near-physiological conditions can be employed to develop ecological hypotheses to understand microbial plant-fiber degradation at the single-cell nanoscale level.

One Sentence Summary This study contributes critical insights into the in-situ organization of cellulosomes and their cellulosic substrates and provides evidence for phenotypic heterogeneity, with dynamic, growth phase-dependent organization of the fiber-degrading machinery.

Competing Interest Statement

The authors have declared no competing interest.

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. All rights reserved. No reuse allowed without permission.
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Posted February 16, 2021.
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Nanoscale resolution of microbial fiber degradation in action
Meltem Tatlı, Sarah Moraïs, Omar E. Tovar-Herrera, Yannick Bomble, Edward A. Bayer, Ohad Medalia, Itzhak Mizrahi
bioRxiv 2021.02.16.431430; doi: https://doi.org/10.1101/2021.02.16.431430
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Nanoscale resolution of microbial fiber degradation in action
Meltem Tatlı, Sarah Moraïs, Omar E. Tovar-Herrera, Yannick Bomble, Edward A. Bayer, Ohad Medalia, Itzhak Mizrahi
bioRxiv 2021.02.16.431430; doi: https://doi.org/10.1101/2021.02.16.431430

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