Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells

Maxence Le Vasseur; Jonathan R. Friedman; Marco Jost; Jiawei Xu; Justin Yamada; Martin Kampmann; Max A. Horlbeck; Michelle R. Salemi; Brett S. Phinney; Jonathan S. Weissman; Jodi Nunnari

doi:10.1101/2021.02.16.431450

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Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells

Maxence Le Vasseur, Jonathan R. Friedman, Marco Jost, Jiawei Xu, Justin Yamada, Martin Kampmann, Max A. Horlbeck, Michelle R. Salemi, Brett S. Phinney, Jonathan S. Weissman, Jodi Nunnari

doi: https://doi.org/10.1101/2021.02.16.431450

Maxence Le Vasseur

1Department of Molecular and Cellular Biology, College of Biological Sciences, University of California, Davis, CA, 95616, USA

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Jonathan R. Friedman

1Department of Molecular and Cellular Biology, College of Biological Sciences, University of California, Davis, CA, 95616, USA

2Department of Cell Biology, University of Texas Southwestern Medical Center, Dallas, TX, 75390, USA

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Marco Jost

3Department of Cellular and Molecular Pharmacology, University of California at San Francisco, San Francisco, CA, 94158, USA

4Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, 94158, USA

5Department of Microbiology and Immunology, University of California at San Francisco, San Francisco, CA, 94158, USA

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Jiawei Xu

1Department of Molecular and Cellular Biology, College of Biological Sciences, University of California, Davis, CA, 95616, USA

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Justin Yamada

1Department of Molecular and Cellular Biology, College of Biological Sciences, University of California, Davis, CA, 95616, USA

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Martin Kampmann

3Department of Cellular and Molecular Pharmacology, University of California at San Francisco, San Francisco, CA, 94158, USA

4Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, 94158, USA

6Institute for Neurodegenerative Diseases and Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA, 94158, USA

7Chan-Zuckerberg Biohub, San Francisco, CA, 94158, USA

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Max A. Horlbeck

3Department of Cellular and Molecular Pharmacology, University of California at San Francisco, San Francisco, CA, 94158, USA

4Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, 94158, USA

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Michelle R. Salemi

8Proteomics Core Facility, University of California, Davis, CA 95616, USA

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Brett S. Phinney

8Proteomics Core Facility, University of California, Davis, CA 95616, USA

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Jonathan S. Weissman

3Department of Cellular and Molecular Pharmacology, University of California at San Francisco, San Francisco, CA, 94158, USA

4Howard Hughes Medical Institute, University of California at San Francisco, San Francisco, CA, 94158, USA

9Whitehead Institute, Cambridge, MA, USA

10Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA

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Jodi Nunnari

1Department of Molecular and Cellular Biology, College of Biological Sciences, University of California, Davis, CA, 95616, USA

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For correspondence: jmnunnari@ucdavis.edu

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Source data 1[supplements/431450_file03.txt]
Source data 2[supplements/431450_file04.txt]
Source data 3[supplements/431450_file05.csv]
Source data 4[supplements/431450_file06.csv]
Supplemental table 1[supplements/431450_file07.xlsx]

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Posted February 17, 2021.

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Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells

Maxence Le Vasseur, Jonathan R. Friedman, Marco Jost, Jiawei Xu, Justin Yamada, Martin Kampmann, Max A. Horlbeck, Michelle R. Salemi, Brett S. Phinney, Jonathan S. Weissman, Jodi Nunnari

bioRxiv 2021.02.16.431450; doi: https://doi.org/10.1101/2021.02.16.431450

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Genome-wide CRISPRi screening identifies OCIAD1 as a prohibitin client and regulatory determinant of mitochondrial Complex III assembly in human cells

Maxence Le Vasseur, Jonathan R. Friedman, Marco Jost, Jiawei Xu, Justin Yamada, Martin Kampmann, Max A. Horlbeck, Michelle R. Salemi, Brett S. Phinney, Jonathan S. Weissman, Jodi Nunnari

bioRxiv 2021.02.16.431450; doi: https://doi.org/10.1101/2021.02.16.431450

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